Target-regulated Ag2S/FeOOH heterojunction activity: a direct label-free photoelectrochemical immunosensor

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL Microchimica Acta Pub Date : 2025-01-18 DOI:10.1007/s00604-024-06929-6

Rui-Jin Shao, Min-Li Wang, Yadong Xue, Na Zhang, Ai-Jun Wang, Pei Song, Li-Ping Mei, Jiu-Ju Feng

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Abstract

Myoglobin (Mb), an important cardiac marker, plays a crucial role in diagnosing, monitoring, and evaluating the condition of patients with cardiovascular diseases. Here, we propose a label-free photoelectrochemical (PEC) sensor for the detection of Mb through target regulated the photoactivity of Ag₂S/FeOOH heterojunction. The Ag₂S/FeOOH nanospindles were synthesized and served as a sensing platform for the fabrication of bio-recognized process for Mb. Mb-aptamer was used as the responsive group to grasp the target Mb in a real sample due to its advantages of strong affinity, high stability, and ease of preparation. Mb-aptamer immunocomplex is formed in the presence of Mb, which hinders the interfacial electron transfer and then reduce the photocurrent. The proposed PEC aptasensor exhibited excellent analytical performance including wide linear range (1.0 pg mL⁻¹ ~ 50 ng mL⁻¹), low limit of detection (0.28 pg mL⁻¹), and good selectivity and stability. This work introduces an innovative approach to PEC aptasensor, offering a promising method for precise determination of human biomarkers.

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靶调控的Ag2S/FeOOH异质结活性：一种直接无标记的光电化学免疫传感器

肌红蛋白（Mb）是一种重要的心脏标志物，在心血管疾病患者的诊断、监测和评估中起着至关重要的作用。在这里，我们提出了一种无标记的光电化学（PEC）传感器，通过靶调节Ag2S/FeOOH异质结的光活性来检测Mb。合成了Ag2S/FeOOH纳米纺锤体，作为构建Mb生物识别过程的传感平台。Mb-aptamer具有亲和力强、稳定性高、制备简单等优点，可作为响应基团在真实样品中捕获目标Mb。Mb-适体免疫复合物在Mb存在下形成，阻碍了界面电子转移，从而降低了光电流。该传感器线性范围宽（1.0 pg mL−1 ~ 50 ng mL−1），检出限低（0.28 pg mL−1），具有良好的选择性和稳定性。这项工作介绍了一种创新的PEC配体传感器方法，为精确测定人类生物标志物提供了一种有前途的方法。图形抽象

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.