Cell-free supernatants from plant growth-promoting rhizobacteria Bacillus albus strains control Aspergillus flavus disease in peanut and maize seedlings

IF 2.5 Q2 MULTIDISCIPLINARY SCIENCES Beni-Suef University Journal of Basic and Applied Sciences Pub Date : 2025-01-17 DOI:10.1186/s43088-025-00594-1

Lai Loi Trinh, Kim Ngoc Le, Hoang Anh Le Lam, Hoai Huong Nguyen

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Abstract

Background

Aspergillus flavus, a seed-borne fungal pathogen, colonizes host plants and exploits nutrients, hindering the growth of seedlings such as peanut and maize. This study investigates the effectiveness of cell-free supernatants (CFSs) from the plant growth-promoting rhizobacteria (PGPR) Bacillus albus strains NNK24 and NDP61, which belong to the Bacillus cereus group, in suppressing A. flavus AF1.

Results

The antifungal activity of these CFSs was attributed to their surfactant properties and chemical composition. These were characterized using rapid chemical assays and ultra-high-performance liquid chromatography-electrospray ionization-quadrupole time-of-flight/mass spectrometry (UHPLC-ESI-QTOF/MS), combined with bioinformatic tools such as Global Natural Product Social Molecular Networking (GNPS) and Natural Products Atlas (NPAtlas). Identified putative antifungal compounds included two diketopiperazines (cyclo(Pro-Leu) and cyclo(2-hydroxy-Pro-Leu)), four macrolactins (7-O-succinyl macrolactin A, 7-O-methyl-5′-hydroxy-3′-heptenoate-macrolactin, macrolactin B, and macrolactin C), two siderophores (petrobactin and bacillibactin), and three cyclic lipopeptides (kurstakin 1, 2 or 3, and 4). These compounds are hypothesized to act synergistically via multiple mechanisms, including disruption of fungal membranes, iron capture, direct antibiosis, and triggering plant immunity. Both CFSs strongly suppressed the harmful effects of A. flavus AF1 and seed-borne A. flavus on peanut and maize seedlings, reducing disease incidence (DI) and disease severity index (DSI) compared to controls. The disease control efficacy (DCE) of the CFSs was comparable to that of the commercial fungicide. Additionally, the CFSs enhanced seed germination, vigor, seedling length, and weight in both peanut and maize. Vigor index (VI) values increased by 222.4–286.0% in peanuts and 181.7–216.4% in maize at 7 days after treatment (DAT).

Conclusion

CFSs of B. albus NNK24 and NDP61 show significant potential as bioprotective agents for sustainable agriculture. Importantly, their use eliminates the need for live bacterial cells from the B. cereus group, addressing biosafety concerns.

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促进植物生长的白色芽孢杆菌的无细胞上清液控制花生和玉米幼苗黄曲霉病

黄曲霉是一种种子传播的真菌病原体，它在寄主植物中定植并利用营养物质，阻碍花生和玉米等幼苗的生长。本研究研究了蜡样芽孢杆菌属植物促生长根瘤菌（PGPR）白芽孢杆菌（Bacillus albus）菌株NNK24和NDP61的无细胞上清液（CFSs）对黄曲霉AF1的抑制作用。结果这些CFSs的抗真菌活性与它们的表面活性剂性质和化学成分有关。利用快速化学分析和超高效液相色谱-电喷雾电离-四极杆飞行时间/质谱（UHPLC-ESI-QTOF/MS），结合全球天然产物社会分子网络（GNPS）和天然产物图谱（NPAtlas）等生物信息学工具，对这些产品进行了表征。已知的抗真菌化合物包括两种二酮哌嗪类化合物（环（原- leu）和环（2-羟基-原- leu）），四种大乳酸素（7- o-琥珀酰大乳酸素A， 7- o-甲基-5 ' -羟基-3 ' - heptenate -大乳酸素，大乳酸素B和大乳酸素C），两种铁载体（油杆菌素和杆菌素）和三种环脂肽（kurstakin 1, 2或3和4）。这些化合物被假设通过多种机制协同作用，包括破坏真菌膜，铁捕获，直接抗生素，并触发植物免疫。与对照相比，两种CFSs均能有效抑制黄曲霉AF1和种传黄曲霉对花生和玉米幼苗的危害，降低病害发生率（DI）和病害严重程度指数（DSI）。CFSs的防病效果（DCE）与市售杀菌剂相当。此外，CFSs对花生和玉米的种子萌发、活力、幼苗长度和幼苗质量均有促进作用。处理后7 d，花生活力指数（VI）提高222.4 ~ 286.0%，玉米活力指数（VI）提高181.7 ~ 216.4%。结论白念珠菌NNK24和NDP61的cfss在农业可持续发展中具有较大的应用潜力。重要的是，它们的使用消除了对蜡样芽孢杆菌群活细菌细胞的需求，解决了生物安全问题。图形抽象

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来源期刊

Beni-Suef University Journal of Basic and Applied Sciences MULTIDISCIPLINARY SCIENCES-

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2.60

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0.00%

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期刊介绍： Beni-Suef University Journal of Basic and Applied Sciences (BJBAS) is a peer-reviewed, open-access journal. This journal welcomes submissions of original research, literature reviews, and editorials in its respected fields of fundamental science, applied science (with a particular focus on the fields of applied nanotechnology and biotechnology), medical sciences, pharmaceutical sciences, and engineering. The multidisciplinary aspects of the journal encourage global collaboration between researchers in multiple fields and provide cross-disciplinary dissemination of findings.