Lab-on-paper for molecular testing with USB-powered isothermal amplification and fluidic control

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL Microchimica Acta Pub Date : 2025-01-17 DOI:10.1007/s00604-024-06889-x

Hyun Pyo Kim, Ji-Ho Park, Min-Gon Kim, Youngung Seok

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Abstract

The global healthcare market increasingly demands affordable molecular diagnostics for field testing. To address this need, we introduce a lab-on-paper (LOP) platform that integrates isothermal amplification with a specially designed paper strip for molecular testing through an automated microfluidics process. The LOP system is engineered for rapid, cost-effective, and highly sensitive detection, using USB-powered thermal management and a wax valve mechanism. This innovative platform provides an accessible solution for the rapid and accurate detection of various microorganisms, proving particularly advantageous for point-of-care testing in resource-limited environments. Experiments conducted in this study demonstrated the efficacy of the LOP platform in the colorimetric detection of foodborne pathogens. It reliably detected Vibrio vulnificus at concentrations as low as 120 CFU/mL and Salmonella Typhimurium at 60 CFU/mL, with results observable to the naked eye. The entire process, encompassing amplification and detection, was completed within 30 min, underscoring the system’s rapid diagnostic capability. Furthermore, with an assay cost of 5.2 USD per test, the platform offers a highly cost-effective solution for molecular diagnostics, particularly in resource-limited settings. The LOP platform’s portability, ease of use, and affordability make it a promising alternative for various diagnostic applications, including infectious disease monitoring and ensuring food safety.

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实验室纸上分子测试与usb供电等温放大和流体控制

全球医疗保健市场越来越需要负担得起的分子诊断用于现场测试。为了满足这一需求，我们推出了一种纸上实验室（LOP）平台，该平台将等温扩增与专门设计的试纸条集成在一起，通过自动化微流体过程进行分子测试。LOP系统采用usb供电的热管理和蜡阀机构，可实现快速、经济、高灵敏度的检测。这一创新平台为快速准确地检测各种微生物提供了一种可行的解决方案，尤其适用于资源有限环境中的即时检测。本研究的实验证明了LOP平台在食源性致病菌比色检测中的有效性。它能可靠地检测出低至120 CFU/mL的创伤弧菌和60 CFU/mL的鼠伤寒沙门氏菌，结果肉眼可见。整个过程，包括扩增和检测，在30分钟内完成，强调了系统的快速诊断能力。此外，由于每次检测的检测成本为5.2美元，该平台为分子诊断提供了极具成本效益的解决方案，特别是在资源有限的环境中。LOP平台的便携性、易用性和可负担性使其成为各种诊断应用的有希望的替代方案，包括传染病监测和确保食品安全。图形抽象

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.