Probing the Self-Aggregation of l-Tryptophan into Spherical Microstructures and Their Selective Interactions with Bilirubin.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2025-01-22 DOI:10.1021/acsabm.4c01486
Aarya, Anna Sebastian, Kavya P, Supratik Sen Mojumdar
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Abstract

The aggregation of proteins, peptides and amino acids has been a keen subject of interest owing to their implications in metabolic disorders. In this work, we investigated the self-aggregation of the unmodified aromatic amino acid l-tryptophan (Trp) into unusual spherical microstructures. Using fluorescence spectroscopy and field emission scanning electron microscopy (FE-SEM), we detail the time-dependent transformation of monomeric tryptophan into spherical aggregates with distinct fluorescence characteristics (λex = 345 nm, λem = 430 nm) compared to the monomer. Notably, the fluorescence intensity of these aggregates is selectively quenched in the presence of bilirubin, demonstrating exceptional sensitivity in the picomolar concentration range. The developed assay proved applicable and reliable for real sample analysis. Thermodynamic parameters derived from temperature-dependent fluorescence intensity measurements indicated that the aggregation process is spontaneous and driven by noncovalent interactions. Further evidence of bilirubin's strong association with the aggregates was obtained through competitive interaction studies with human serum albumin (HSA). This work offers insights into the aggregation behavior of single aromatic amino acids and their potential applications in detecting critical analytes.

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探索l-色氨酸自聚集成球形微观结构及其与胆红素的选择性相互作用。
由于蛋白质、多肽和氨基酸的聚集与代谢紊乱有关,它们一直是人们感兴趣的一个重要课题。在这项工作中,我们研究了未经修饰的芳香氨基酸l-色氨酸(Trp)自聚集成不寻常的球形微观结构。利用荧光光谱和场发射扫描电镜(FE-SEM),我们详细描述了色氨酸单体转化为具有不同荧光特征(λex = 345 nm, λem = 430 nm)的球形聚集体的时间依赖性。值得注意的是,这些聚集体的荧光强度在胆红素存在下被选择性猝灭,在皮摩尔浓度范围内表现出异常的敏感性。结果表明,该方法适用于实际样品分析。从温度依赖的荧光强度测量得出的热力学参数表明,聚集过程是自发的,由非共价相互作用驱动。通过与人血清白蛋白(HSA)的竞争性相互作用研究获得了胆红素与聚集体强关联的进一步证据。这项工作提供了对单个芳香氨基酸的聚集行为及其在检测关键分析物中的潜在应用的见解。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
期刊介绍: ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.
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