A synthetic biology approach for identifying de-SUMOylation enzymes of substrates.

IF 9.3 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Journal of Integrative Plant Biology Pub Date : 2025-01-21 DOI:10.1111/jipb.13838
Junwen Huang, Junjie Huang, Jiayuan Wu, Mi Zhou, Siyi Luo, Jieming Jiang, Tongsheng Chen, Ling Shao, Jianbin Lai, Chengwei Yang
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Abstract

A synthetic biology approach using a robust reconstitution system in Escherichia coli enables the identification of plant ubiquitin-like proteases responsible for removing the small ubiquitin-like modifier (SUMO) post-translational modifications from specific protein substrates.

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鉴定底物去苏酰化酶的合成生物学方法。
一种在大肠杆菌中使用强大重组系统的合成生物学方法能够鉴定植物泛素样蛋白酶,该蛋白酶负责从特定蛋白质底物中去除小泛素样修饰物(SUMO)翻译后修饰。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Integrative Plant Biology
Journal of Integrative Plant Biology 生物-生化与分子生物学
CiteScore
18.00
自引率
5.30%
发文量
220
审稿时长
3 months
期刊介绍: Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.
期刊最新文献
Regulatory and retrograde signaling networks in the chlorophyll biosynthetic pathway. PSC1, a basic/helix-loop-helix transcription factor controlling the purplish-red testa trait in peanut. A synthetic biology approach for identifying de-SUMOylation enzymes of substrates. CircZmMED16 delays plant flowering by negatively regulating starch content through its binding to ZmAPS1. ALBA3 maintains male fertility under heat stress in plants.
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