Bacillus subtilis MZ-01 alleviates diarrhea caused by ETEC K88 by reducing inflammation and promoting intestinal health.

IF 3.2 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal of Applied Microbiology Pub Date : 2025-01-16 DOI:10.1093/jambio/lxaf018

Jianwei Qin, Yang Liu, Mingming Cao, Yue Zhang, Guangdong Bai, Baoming Shi

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Abstract

Aims: The purpose of this study was to investigate the effects of Bacillus subtilis supplementation on the health of weaned piglets and whether Bacillus subtilis supplementation can reduce the damage of piglets induced by ETEC K88.

Methods and results: The experiment was designed with a 2 × 2 factorial arrangement, comprising the control (CON) group, Bacillus subtilis (PRO) group, Escherichia coli K88 (ETEC) group, and Bacillus subtilis + ETEC (PRO + ETEC) group. Regardless of the presence of ETEC, the addition of PRO increased the piglets' final body weight (FW), average daily gain (ADG), and daily feed intake (ADFI). Additionally, PRO primarily achieves a reduction in heat-stable enterotoxin (ST) levels, suppresses the expression of NF-κB, TLR4, and MyD88 mRNA in the jejunum and ileum, lowers pro-inflammatory factors in the blood and small intestine, enhances the expression of tight junction proteins in the small intestine, improves the composition of the colonic microbiota, increases colonic short-chain fatty acids (SCFAs) contents, thereby alleviates diarrhea and mitigates bodily damage caused by ETEC K88 infection.

Conclusion: The addition of Bacillus subtilis MZ-01 alleviated ETEC K88-induced piglet diarrhoea by reducing heat-stable enterotoxin levels, decreasing pro-inflammatory factors in the blood and intestine, and enhancing the intestinal barrier and tight junction proteins.

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枯草芽孢杆菌MZ-01可减轻ETEC K88引起的腹泻，减少炎症，促进肠道健康。

目的：研究饲粮中添加枯草芽孢杆菌对断奶仔猪健康的影响，以及是否能减轻ETEC K88对仔猪的损伤。方法与结果：采用2 × 2因子设计，分为对照（CON）组、枯草芽孢杆菌（PRO）组、大肠杆菌K88 （ETEC）组和枯草芽孢杆菌+ ETEC （PRO + ETEC）组。无论ETEC是否存在，添加PRO均能提高仔猪最终体重（FW）、平均日增重（ADG）和日采食量（ADFI）。此外，PRO主要实现降低热稳定性肠毒素（ST）水平，抑制空肠和回肠中NF-κB、TLR4和MyD88 mRNA的表达，降低血液和小肠中的促炎因子，增强小肠紧密连接蛋白的表达，改善结肠微生物群的组成，增加结肠短链脂肪酸（SCFAs）含量。从而减轻腹泻和减轻由ETEC K88感染引起的身体损害。结论：添加枯草芽孢杆菌MZ-01可通过降低热稳定型肠毒素水平、降低血液和肠道促炎因子、增强肠道屏障和紧密连接蛋白来缓解ETEC k88诱导仔猪腹泻。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Applied Microbiology 生物-生物工程与应用微生物

CiteScore

7.30

自引率

2.50%

发文量

427

审稿时长

2.7 months

期刊介绍： Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.