Unveiling the Molecular Mechanisms of Rosacea: Insights From Transcriptomics and In Vitro Experiments

Abstract

Background

Rosacea is a prevalent inflammatory skin condition, but its molecular mechanisms and treatment responses remain poorly understood.

Aims

This study aims to investigate the molecular mechanisms underlying rosacea and explore drug response through transcriptomic analysis and in vitro experiments.

Patients/Methods

We performed high-throughput RNA sequencing to analyze gene expression patterns in rosacea patients. In vitro experiments, including RT-qPCR, Western blot, ELISA, scratch, and Transwell assays, were used to evaluate gene and protein expression and cell behavior in HaCaT cells under simulated rosacea conditions.

Results

Transcriptomic analysis revealed significantly elevated expression of inflammatory-related genes in rosacea patients. In vitro, HaCaT cells exhibited enhanced proliferation and migration abilities, accompanied by increased expression of pro-inflammatory genes and proteins. Specifically, Toll-like receptor 2 (TLR2) and S100A9 proteins were upregulated, potentially promoting these processes.

Conclusions

Our study elucidates the molecular mechanisms of rosacea, highlighting the role of inflammatory pathways and altered cell behavior in the disease. TLR2 and S100A9 may contribute to disease progression, offering potential targets for future therapeutic strategies.