Cyto-Genotoxic Assessment of Sulfoxaflor in Allium cepa Root Cells and DNA Docking Studies.

Abstract

Sulfoxaflor (SFX) is an insecticide that is commonly used for the control of sap-feeding insects. Since SFX is extensively applied globally, it has been implicated in the substantial induction of environmental toxicity. Therefore, in this study, Allium cepa roots have been employed to elucidate the potential cytogenotoxic effects of SFX in non-target cells by examination of mitotic index (MI), chromosomal aberrations (CAs), and DNA damage. Physiological effects of SFX were evaluated by A. cepa root growth inhibition assay, while cytogenotoxic effects were assessed by A. cepa ana-telophase and comet assay. Moreover, DNA binding affinity and binding mode of SFX were examined using molecular docking simulations to shed light on the genotoxic mechanism of action. The half maximal effective concentration (EC₅₀) on the growth of A. cepa cells calculated for SFX was found as 500 mg/L. Moreover, dose- and time-dependent decrease in MI, increase in CAs (disturbed ana-telophase, chromosomal laggards, stickiness, and anaphase chromosome bridge) and DNA damage were observed by the exposure of A. cepa root tips to SFX after 24-, 48-, 72-, and 96-h treatment periods. A 6-bp double-stranded DNA structure containing two intercalation sites (PDB ID: 1Z3F) was used for docking studies. According to DNA docking results, SFX exhibited an energetically more favorable binding affinity with DNA (ΔG = -5.05 kcal/mol) compared with the experimental mutagen methyl methanesulfonate (MMS) (ΔG = -2.94 kcal/mol), and preferentially snugly fits into the minor groove of DNA possessing an intercalation gap, thus, providing valuable mechanistic data into the formation of chromosome aberrations and DNA fragmentation induced by this pesticide in A. cepa.