Weihang Geng, Qiming Xia, Mubin He, Yi Wang, Zheng Zheng, Haiyan Qin, Yalun Wang, Jian Feng, Jun Qian
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引用次数: 0
Abstract
Three-photon fluorescence (3PF) microscopy encounters significant challenges in biological research and clinical applications, primarily due to the limited availability of high-performance probes. We took a shortcut by exploring the excellent 3PF property of berberine hydrochloride (BH), a clinically utilized drug derived from the traditional Chinese medicine, Coptis. Capitalizing on its renal metabolism characteristics, we employed BH for in vivo 3PF microscopic imaging of the mouse kidney. This approach enabled high-resolution structural observation of renal tubules and facilitated the diagnosis of drug-induced acute kidney injury (AKI) based on renal tubule morphology. The analytical capabilities of 3PF microscopy for renal physiology and pathological diagnosis suggest its potential for future clinical applications.
期刊介绍：
The first international journal dedicated to publishing reviews and original articles from this exciting field, the Journal of Biophotonics covers the broad range of research on interactions between light and biological material. The journal offers a platform where the physicist communicates with the biologist and where the clinical practitioner learns about the latest tools for the diagnosis of diseases. As such, the journal is highly interdisciplinary, publishing cutting edge research in the fields of life sciences, medicine, physics, chemistry, and engineering. The coverage extends from fundamental research to specific developments, while also including the latest applications.
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