Comparison of urinary cortisol, urinary cortisol-to-creatinine ratio, and basal serum cortisol as screening tests for hypoadrenocorticism in dogs.

IF 1.4 3区农林科学 Q2 VETERINARY SCIENCES American journal of veterinary research Pub Date : 2025-01-21 Print Date: 2025-03-01 DOI:10.2460/ajvr.24.10.0296

Federico Fracassi, Alessandro Tirolo, Matteo Galeotti, Andrea Corsini, Andrea Bertolazzi, Amtonio M Tardo, Stefania Golinelli, Walter Bertazzolo, Ugo Bonfanti, Fabio Procoli, Francesca Del Baldo

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Abstract

Objective: This study investigates whether urinary cortisol (UC) and UC-to-creatinine ratio (UCCR) perform better than basal serum cortisol (BSC) in identifying dogs with hypoadrenocorticism (HA).

Methods: A retrospective, multicenter study with 120 client-owned dogs included: 20 with HA, 42 healthy, and 60 with diseases mimicking HA. The UC and UCCR were determined on urine samples using a chemiluminescent enzyme immunoassay. The diagnostic performance of the UC and UCCR were assessed based on receiver operating characteristics (ROC) curves.

Results: A cutoff value of UC < 2 µg/dL revealed 100% sensitivity (95% CI, 83.2 to 100) and 90.0% specificity (95% CI, 79.5 to 96.2) in diagnosing HA. A cutoff value of UCCR < 8.5 X 10-6 revealed 100% sensitivity (95% CI, 83.1 to 100) and 71.7% specificity (95% CI, 58.6 to 82.6) in diagnosing HA. A cutoff value of BSC < 2 µg/dL and < 1 µg/dL revealed 100% sensitivity (95% CI, 83.2 to 100) and 51.7% specificity (95% CI, 38.5 to 64.8) and 100% sensitivity (95% CI, 83.9 to 100) and 90% specificity (95% CI, 79.8 to 95.3) in diagnosing HA, respectively.

Conclusions: BSC < 1 µg/dL showed the same sensitivity but higher specificity than BSC < 2 µg/dL. The UC < 2 µg/dL showed noninferior performance compared with the BSC < 1 µg/dL.

Clinical relevance: UC should be considered a promising screening test for canine HA.

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尿皮质醇、尿皮质醇-肌酐比值和基础血清皮质醇作为犬肾上腺皮质减退症筛查试验的比较。

目的：研究尿皮质醇（UC）和UC /肌酐比值（UCCR）是否比基础血清皮质醇（BSC）更能识别肾上腺皮质减退症（HA）。方法：对120只客户拥有的狗进行回顾性多中心研究，其中20只患有HA， 42只健康，60只患有类似HA的疾病。用化学发光酶免疫分析法测定尿液样品的UC和UCCR。根据受试者工作特征（ROC）曲线评估UC和UCCR的诊断效能。结果：UC < 2µg/dL的临界值显示诊断HA的100%敏感性（95% CI, 83.2 ~ 100）和90.0%特异性（95% CI, 79.5 ~ 96.2）。UCCR < 8.5 × 10-6的临界值显示诊断HA的敏感性为100% (95% CI, 83.1 ~ 100)，特异性为71.7% （95% CI, 58.6 ~ 82.6）。BSC < 2µg/dL和< 1µg/dL的临界值分别显示诊断HA的100%敏感性（95% CI, 83.2 ~ 100）和51.7%特异性（95% CI, 38.5 ~ 64.8）， 100%敏感性（95% CI, 83.9 ~ 100）和90%特异性（95% CI, 79.8 ~ 95.3）。结论：BSC < 1µg/dL与BSC < 2µg/dL具有相同的敏感性和更高的特异性。与BSC < 1µg/dL相比，UC < 2µg/dL表现出不逊色的性能。临床相关性：UC应该被认为是一种很有前途的犬HA筛查试验。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

American journal of veterinary research 农林科学-兽医学

CiteScore

1.70

自引率

10.00%

发文量

186

审稿时长

3 months

期刊介绍： The American Journal of Veterinary Research supports the collaborative exchange of information between researchers and clinicians by publishing novel research findings that bridge the gulf between basic research and clinical practice or that help to translate laboratory research and preclinical studies to the development of clinical trials and clinical practice. The journal welcomes submission of high-quality original studies and review articles in a wide range of scientific fields, including anatomy, anesthesiology, animal welfare, behavior, epidemiology, genetics, heredity, infectious disease, molecular biology, oncology, pharmacology, pathogenic mechanisms, physiology, surgery, theriogenology, toxicology, and vaccinology. Species of interest include production animals, companion animals, equids, exotic animals, birds, reptiles, and wild and marine animals. Reports of laboratory animal studies and studies involving the use of animals as experimental models of human diseases are considered only when the study results are of demonstrable benefit to the species used in the research or to another species of veterinary interest. Other fields of interest or animals species are not necessarily excluded from consideration, but such reports must focus on novel research findings. Submitted papers must make an original and substantial contribution to the veterinary medicine knowledge base; preliminary studies are not appropriate.