{"title":"Chemogenetic modulation of parathyroid hormone secretion alleviates osteoporosis in ovariectomized rats","authors":"Yutong Xiao , Qian Yuan , Fan Yang , Yunhui Liu","doi":"10.1016/j.bbrc.2025.151362","DOIUrl":null,"url":null,"abstract":"<div><div>Parathyroid hormone (PTH) is critical for regulating calcium and phosphate homeostasis, and its dysregulation contributes to osteoporosis. Current methods for precise control of PTH secretion are limited. This study explores chemogenetic tools to regulate PTH secretion in parathyroid chief cells via Gq/Gi signaling. In vitro, we found that activation of hM3Dq-expressing cells increased PTH release, while hM4Di inhibited it. In vivo, hM3Dq activation improved bone structure and reduced bone loss in an ovariectomized rat model. These findings suggest chemogenetics as a promising approach for modulating PTH and offering potential therapeutic strategies for bone health and related disorders.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"749 ","pages":"Article 151362"},"PeriodicalIF":2.5000,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemical and biophysical research communications","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0006291X25000762","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Parathyroid hormone (PTH) is critical for regulating calcium and phosphate homeostasis, and its dysregulation contributes to osteoporosis. Current methods for precise control of PTH secretion are limited. This study explores chemogenetic tools to regulate PTH secretion in parathyroid chief cells via Gq/Gi signaling. In vitro, we found that activation of hM3Dq-expressing cells increased PTH release, while hM4Di inhibited it. In vivo, hM3Dq activation improved bone structure and reduced bone loss in an ovariectomized rat model. These findings suggest chemogenetics as a promising approach for modulating PTH and offering potential therapeutic strategies for bone health and related disorders.
期刊介绍：
Biochemical and Biophysical Research Communications is the premier international journal devoted to the very rapid dissemination of timely and significant experimental results in diverse fields of biological research. The development of the "Breakthroughs and Views" section brings the minireview format to the journal, and issues often contain collections of special interest manuscripts. BBRC is published weekly (52 issues/year).Research Areas now include: Biochemistry; biophysics; cell biology; developmental biology; immunology
; molecular biology; neurobiology; plant biology and proteomics
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