PIASy of orange-spotted grouper (Epinephelus coioides) negatively regulates RLRs-mediated innate antiviral immunity

IF 3.9 2区农林科学 Q1 FISHERIES Fish & shellfish immunology Pub Date : 2025-03-01 Epub Date: 2025-01-20 DOI:10.1016/j.fsi.2025.110146

Qiongyue Xu , Siting Wu , Xiaoxia Lei , Helong Cao , Zhouling Zhan , Qiwei Qin , Jingguang Wei

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Abstract

During viral infection, RIG-I-like receptors (RLRs) are cytoplasmic pattern recognition receptors that recognize and bind to viral RNA components, initiating the transcription of interferon-related genes, inflammatory cytokines and other factors, thereby triggering the cellular production of an antiviral innate immune response. The protein inhibitor of activated signal transducer and activator of transcription (STAT) (PIAS) protein family has become a hot research topic due to its extensive involvement in the regulation of cytokines, inflammatory factors and innate immune signaling pathways. In the present study, we investigated the role of fish PIASy in Singapore grouper iridovirus (SGIV) and red spotted grouper nervous necrosis virus (RGNNV) infections. The homologous sequence of orange-spotted grouper (Epinephelus coioides) PIASy gene (EcPIASy) was cloned and characterized, which encoded a 498-amino acid protein with 99.20 % homology to Plectropomus leopardus. EcPIASy is expressed mainly in gills, blood, and liver. Subcellular localization showed that EcPIASy was uniformly distributed in the nucleus. Overexpression of EcPIASy promoted SGIV and RGNNV replication, and inhibited the expression of interferon related genes and pro-inflammatory factors induced by viruses. In addition, EcPIASy interacts with RLR signaling pathway-related genes EcMDA5, EcIRF3 and EcIRF7, whereas the interaction between EcPIASy and EcIRF3 does not depend on any specific structural domain of EcPIASy. The results provide a better understanding of the relationship between PIASy and viral infection in fish.

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橙斑石斑鱼（Epinephelus coioides）的PIASy负向调节rlrs介导的先天抗病毒免疫。

在病毒感染过程中，rig - i样受体（rlr）是细胞质模式识别受体，识别并结合病毒RNA成分，启动干扰素相关基因、炎症细胞因子等因子的转录，从而触发细胞产生抗病毒先天免疫反应。活化信号换能器和转录激活因子（activated signal transducer and activator of transcription， STAT）蛋白家族的蛋白抑制剂因其广泛参与细胞因子、炎症因子和先天免疫信号通路的调控而成为研究热点。本研究探讨了PIASy在新加坡石斑鱼虹膜病毒（SGIV）和红斑石斑鱼神经坏死病毒（RGNNV）感染中的作用。克隆并鉴定了斑点石斑鱼（Epinephelus coioides） PIASy基因（EcPIASy）的同源序列，该基因编码一个498个氨基酸的蛋白，与豹斑石斑鱼（plectroomus leopardus）同源性为99.20%。EcPIASy主要在鳃、血液和肝脏中表达。亚细胞定位显示，EcPIASy在细胞核内分布均匀。过表达EcPIASy可促进SGIV和RGNNV复制，抑制病毒诱导的干扰素相关基因和促炎因子的表达。此外，EcPIASy与RLR信号通路相关基因EcMDA5、EcIRF3和EcIRF7相互作用，而EcPIASy与EcIRF3之间的相互作用不依赖于EcPIASy的任何特定结构域。该结果为进一步了解PIASy与鱼类病毒感染之间的关系提供了依据。

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来源期刊

Fish & shellfish immunology 农林科学-海洋与淡水生物学

CiteScore

7.50

自引率

19.10%

发文量

750

审稿时长

68 days

期刊介绍： Fish and Shellfish Immunology rapidly publishes high-quality, peer-refereed contributions in the expanding fields of fish and shellfish immunology. It presents studies on the basic mechanisms of both the specific and non-specific defense systems, the cells, tissues, and humoral factors involved, their dependence on environmental and intrinsic factors, response to pathogens, response to vaccination, and applied studies on the development of specific vaccines for use in the aquaculture industry.