Phospho-Proteomics Analysis of Early Response to X-Ray Irradiation Reveals Molecular Mechanism Potentially Related to U251 Cell Radioresistance.

IF 3.6 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Proteomes Pub Date : 2024-12-25 DOI:10.3390/proteomes13010001

Ousseynou Ben Diouf, Antoine Gilbert, Benoit Bernay, Randi G Syljuåsen, Mihaela Tudor, Mihaela Temelie, Diana I Savu, Mamadou Soumboundou, Cheikh Sall, François Chevalier

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Abstract

Glioblastoma (GBM) is a devastating malignant brain tumor with a poor prognosis. GBM is associated with radioresistance. Post-translational modifications (PTMs) such as protein phosphorylation can play an important role in the cellular response to radiation. To better understand the early cellular activities after radiation in GBM, we carried out a phospho-proteomic study on the U251 cell line 3 h after X-ray irradiation (6Gy) and on non-irradiated cells. Our study showed a strong modification of proteoform phosphorylation in response to radiation. We found 453 differentially expressed phosphopeptides (DEPs), with 211 being upregulated and 242 being downregulated. A GO enrichment analysis of DEPs showed a strong enrichment of the signaling pathways involved in DNA damage response after irradiation and categorized them into biological processes (BPs), cellular components (CCs) and molecular functions (MFs). Certain accessions such as BRCA1, MDC1, H2AX, MDC1, TP53BP1 were dynamically altered in our fraction and are highly associated with the signaling pathways enriched after radiation.

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对x射线辐射早期反应的磷酸化蛋白质组学分析揭示了与U251细胞辐射抗性潜在相关的分子机制

胶质母细胞瘤（GBM）是一种预后不良的恶性脑肿瘤。GBM与辐射抗性有关。翻译后修饰（PTMs）如蛋白质磷酸化可以在细胞对辐射的反应中发挥重要作用。为了更好地了解GBM辐射后的早期细胞活性，我们对U251细胞系在x射线照射（6Gy）后3小时和未照射细胞进行了磷酸化蛋白质组学研究。我们的研究表明，在辐射的作用下，蛋白质的磷酸化发生了强烈的变化。我们发现453个差异表达的磷酸肽（DEPs），其中211个表达上调，242个表达下调。DEPs的氧化石墨烯富集分析显示，辐照后参与DNA损伤反应的信号通路富集，并将其分为生物过程（bp）、细胞成分（CCs）和分子功能（MFs）。某些基因如BRCA1、MDC1、H2AX、MDC1、TP53BP1在我们的片段中发生了动态变化，并且与辐射后信号通路的富集高度相关。

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来源期刊

Proteomes Biochemistry, Genetics and Molecular Biology-Clinical Biochemistry

CiteScore

6.50

自引率

3.00%

发文量

审稿时长

11 weeks

期刊介绍： Proteomes (ISSN 2227-7382) is an open access, peer reviewed journal on all aspects of proteome science. Proteomes covers the multi-disciplinary topics of structural and functional biology, protein chemistry, cell biology, methodology used for protein analysis, including mass spectrometry, protein arrays, bioinformatics, HTS assays, etc. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on the length of papers. Scope: -whole proteome analysis of any organism -disease/pharmaceutical studies -comparative proteomics -protein-ligand/protein interactions -structure/functional proteomics -gene expression -methodology -bioinformatics -applications of proteomics