Effects of photobiomodulation in mitochondrial quantity, biogenesis and mitophagy-associated genes in breast cancer cells.

IF 2.4 4区医学 Q3 ENGINEERING, BIOMEDICAL Lasers in Medical Science Pub Date : 2025-01-24 DOI:10.1007/s10103-025-04287-0

Larissa Alexsandra da Silva Neto Trajano, Priscyanne Barreto Siqueira, Daphne Pinheiro, Thayssa Gomes Farias, Márcia Soares Dos Santos, Bruno Ricardo Barreto Pires, Adenilson de Souza da Fonseca, Andre Luiz Mencalha

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Abstract

In this article, we aim to evaluate the effects of photobiomodulation on mitochondria quantity, biogenesis, and mitophagy-associated genes in breast cancer (BC) cells. Both models were irradiated with a low-power infrared laser (880 nm, 150 mW) and amber LED (617 nm, 1500 mW), alone or simultaneously. We evaluated the mRNA expression of PINK1 and PGC-1α genes, and the mitochondrial number was assessed based on the ratio of mitochondrial DNA/genomic DNA (mtDNA/gDNA). No significant difference was observed in the mtDNA/gDNA ratio comparing the low-power infrared laser (LPIL) and LED-irradiated groups to their control counterparts. Similarly, no difference was observed in the mRNA levels of PINK1 and PGC-1α of the irradiated group with an LPIL and LED alone or in combination. In conclusion, PBM with LPIL and LED did not alter the mtDNA/gDNA ratio nor modulate the mRNA levels of the genes related to mitophagy and biogenesis in BC cells.

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光生物调节对乳腺癌细胞线粒体数量、生物发生及线粒体自噬相关基因的影响。

在本文中，我们旨在评估光生物调节对乳腺癌细胞中线粒体数量、生物发生和线粒体自噬相关基因的影响。两种模型分别接受低功率红外激光（880 nm, 150 mW）和琥珀色LED （617 nm, 1500 mW）单独或同时照射。我们评估了PINK1和PGC-1α基因的mRNA表达，并根据线粒体DNA/基因组DNA （mtDNA/gDNA）的比值评估了线粒体数量。低功率红外激光（LPIL）和led照射组与对照组相比，mtDNA/gDNA比率无显著差异。同样，LPIL和LED单独或联合照射组的PINK1和PGC-1α mRNA水平也没有差异。综上所述，含LPIL和LED的PBM不会改变BC细胞mtDNA/gDNA比值，也不会调节与线粒体自噬和生物发生相关基因的mRNA水平。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Lasers in Medical Science 医学-工程：生物医学

CiteScore

4.50

自引率

4.80%

发文量

192

审稿时长

3-8 weeks

期刊介绍： Lasers in Medical Science (LIMS) has established itself as the leading international journal in the rapidly expanding field of medical and dental applications of lasers and light. It provides a forum for the publication of papers on the technical, experimental, and clinical aspects of the use of medical lasers, including lasers in surgery, endoscopy, angioplasty, hyperthermia of tumors, and photodynamic therapy. In addition to medical laser applications, LIMS presents high-quality manuscripts on a wide range of dental topics, including aesthetic dentistry, endodontics, orthodontics, and prosthodontics. The journal publishes articles on the medical and dental applications of novel laser technologies, light delivery systems, sensors to monitor laser effects, basic laser-tissue interactions, and the modeling of laser-tissue interactions. Beyond laser applications, LIMS features articles relating to the use of non-laser light-tissue interactions.