Lysosomal-targeted fluorescent probe for detecting SO2 derivatives in human umbilical vein endothelial cells

IF 4.6 2区 化学 Q1 SPECTROSCOPY Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-04-15 Epub Date: 2025-01-15 DOI:10.1016/j.saa.2025.125753
Feng Li , Xian-Fang Liu , Bao-Xiang Zhao , Zhao-Min Lin
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Abstract

A fluorescent probe (NBC), constructed by benzothiazole-coumarin and naphthalimide derivatives, was developed for the detection of SO2 derivatives using the FRET (Förster Resonance Energy Transfer) strategy. NBC presented large Stokes shift (180 nm), fast response (2 min), high sensitivity (LOD: 45 nM) and an excellent linear relationship in response to SO2 derivatives. Moreover, NBC has been successfully applied to detect SO2 derivatives in food samples and living cells. NBC holds significant potential for monitoring changes in both exogenous and endogenous SO2 derivatives in human umbilical vein endothelial cells (HUVEC cells). Additionally, it demonstrates remarkable lysosomal-targeting capabilities, allowing for the observation of dynamic alterations within lysosomes.

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溶酶体靶向荧光探针检测人脐静脉内皮细胞中SO2衍生物。
以苯并噻唑-香豆素和萘酰亚胺衍生物为原料,构建了一种荧光探针(NBC),利用FRET (Förster共振能量转移)策略检测SO2衍生物。NBC的Stokes位移大(180 nm),响应速度快(2 min),灵敏度高(LOD: 45 nm),对SO2衍生物的响应具有良好的线性关系。此外,NBC已成功应用于食品样品和活细胞中SO2衍生物的检测。NBC在监测人脐静脉内皮细胞(HUVEC细胞)中外源性和内源性SO2衍生物的变化方面具有重要的潜力。此外,它显示了显著的溶酶体靶向能力,允许观察溶酶体内的动态变化。
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来源期刊
CiteScore
8.40
自引率
11.40%
发文量
1364
审稿时长
40 days
期刊介绍: Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.
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