Feng Li , Xian-Fang Liu , Bao-Xiang Zhao , Zhao-Min Lin
{"title":"Lysosomal-targeted fluorescent probe for detecting SO2 derivatives in human umbilical vein endothelial cells","authors":"Feng Li , Xian-Fang Liu , Bao-Xiang Zhao , Zhao-Min Lin","doi":"10.1016/j.saa.2025.125753","DOIUrl":null,"url":null,"abstract":"<div><div>A fluorescent probe <strong>(NBC)</strong>, constructed by benzothiazole-coumarin and naphthalimide derivatives, was developed for the detection of SO<sub>2</sub> derivatives using the FRET (Förster Resonance Energy Transfer) strategy. <strong>NBC</strong> presented large Stokes shift (180 nm), fast response (2 min), high sensitivity (LOD: 45 nM) and an excellent linear relationship in response to SO<sub>2</sub> derivatives. Moreover, <strong>NBC</strong> has been successfully applied to detect SO<sub>2</sub> derivatives in food samples and living cells. <strong>NBC</strong> holds significant potential for monitoring changes in both exogenous and endogenous SO<sub>2</sub> derivatives in human umbilical vein endothelial cells (HUVEC cells). Additionally, it demonstrates remarkable lysosomal-targeting capabilities, allowing for the observation of dynamic alterations within lysosomes.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"331 ","pages":"Article 125753"},"PeriodicalIF":4.3000,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1386142525000599","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"SPECTROSCOPY","Score":null,"Total":0}
引用次数: 0
Abstract
A fluorescent probe (NBC), constructed by benzothiazole-coumarin and naphthalimide derivatives, was developed for the detection of SO2 derivatives using the FRET (Förster Resonance Energy Transfer) strategy. NBC presented large Stokes shift (180 nm), fast response (2 min), high sensitivity (LOD: 45 nM) and an excellent linear relationship in response to SO2 derivatives. Moreover, NBC has been successfully applied to detect SO2 derivatives in food samples and living cells. NBC holds significant potential for monitoring changes in both exogenous and endogenous SO2 derivatives in human umbilical vein endothelial cells (HUVEC cells). Additionally, it demonstrates remarkable lysosomal-targeting capabilities, allowing for the observation of dynamic alterations within lysosomes.
期刊介绍：
Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science.
The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments.
Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate.
Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to:
Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences,
Novel experimental techniques or instrumentation for molecular spectroscopy,
Novel theoretical and computational methods,
Novel applications in photochemistry and photobiology,
Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
