The Positive Regulatory Effect of DBT on Lipid Metabolism in Postpartum Dairy Cows.

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Metabolites Pub Date : 2025-01-16 DOI:10.3390/metabo15010058

Zheng Zhou, Kang Yong, Zhengzhong Luo, Zhenlong Du, Tao Zhou, Xiaoping Li, Xueping Yao, Liuhong Shen, Shumin Yu, Yixin Huang, Suizhong Cao

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Abstract

Background/objectives: The transition from a non-lactating to a lactating state is a critical period for lipid metabolism in dairy cows. Danggui Buxue Tang (DBT), stimulating energy metabolism, ameliorates diseases related to lipid metabolism disorders and is expected to be an effective supplement for alleviating excessive lipid mobilisation in periparturient dairy cows. This study aimed to investigate the effects of supplemental DBT on serum biochemical indices, faecal microbial communities, and plasma metabolites in dairy cows.

Methods: Thirty cows were randomly divided into three groups: H-DBT group, L-DBT group, and control group. DBT administration was started on the day of calving and continued once daily for seven days. Faecal and blood samples were collected on calving day, 7 days after calving, and 14 days after calving. The levels of serum biochemical indices were measured at three time points in the three groups using commercial kits. Cows in the H-DBT group and control group were selected for metabolome and 16S rRNA amplicon sequencing.

Results: Our research shows that, in dairy cows 7 days postpartum, DBT significantly reduced serum 3-hydroxybutyric acid (BHB) concentrations and the number of cows with BHB concentrations ≥ 1 mmol/L. Additionally, DBT increased serum total cholesterol contents at both 7 and 14 days postpartum. Analysis of the microbiota community showed that DBT modulated the composition and structure of the hindgut microbiota. Metabolomic analysis revealed decreased plasma acetylcarnitine, 2-hydroxybutyric acid, and BHB levels 7 days postpartum, whereas the TCA cycle was enhanced. At 14 days postpartum, DBT altered the plasma bile acid profile, especially glycine-conjugated bile acids, including GCDCA, GUDCA, and GDCA. Correlation analyses showed that the relative abundances of Bacillus, Solibacillus, Dorea, and Romboutsia were strongly correlated with the differential metabolites, which is crucial for the beneficial effects of DBT.

Conclusions: DBT improves energy status and lipid metabolism in postpartum dairy cows by modulating hindgut microbiota and serum lipid metabolites.

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DBT对产后奶牛脂质代谢的正向调节作用。

背景/目的：奶牛从非泌乳状态过渡到泌乳状态是脂质代谢的关键时期。当归补血汤（DBT）具有促进能量代谢、改善脂质代谢紊乱相关疾病的作用，有望成为缓解围产期奶牛脂质过度动员的有效补充剂。本试验旨在研究饲粮中添加DBT对奶牛血清生化指标、粪便微生物群落和血浆代谢产物的影响。方法：30头奶牛随机分为3组：H-DBT组、L-DBT组和对照组。从产犊当天开始给药，每天1次，连续7天。分别于产犊日、产犊后第7天和第14天采集粪便和血液样本。采用市售试剂盒检测三组患者三个时间点血清生化指标水平。选择H-DBT组和对照组奶牛进行代谢组和16S rRNA扩增子测序。结果：我们的研究表明，在奶牛产后7天，DBT显著降低了血清3-羟基丁酸（BHB）浓度和BHB浓度≥1 mmol/L的奶牛数量。此外，DBT增加了产后7天和14天的血清总胆固醇含量。微生物群落分析表明，DBT调节了后肠菌群的组成和结构。代谢组学分析显示，产后7天血浆乙酰肉碱、2-羟基丁酸和BHB水平下降，而TCA周期增强。产后14天，DBT改变血浆胆汁酸谱，尤其是甘氨酸偶联胆汁酸，包括GCDCA、GUDCA和GDCA。相关分析表明，芽孢杆菌、固体芽孢杆菌、Dorea和Romboutsia的相对丰度与差异代谢物密切相关，这是DBT有益效果的关键。结论：DBT通过调节后肠道菌群和血清脂质代谢产物改善产后奶牛的能量状态和脂质代谢。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Metabolites Biochemistry, Genetics and Molecular Biology-Molecular Biology

CiteScore

5.70

自引率

7.30%

发文量

1070

审稿时长

17.17 days

期刊介绍： Metabolites (ISSN 2218-1989) is an international, peer-reviewed open access journal of metabolism and metabolomics. Metabolites publishes original research articles and review articles in all molecular aspects of metabolism relevant to the fields of metabolomics, metabolic biochemistry, computational and systems biology, biotechnology and medicine, with a particular focus on the biological roles of metabolites and small molecule biomarkers. Metabolites encourages scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on article length. Sufficient experimental details must be provided to enable the results to be accurately reproduced. Electronic material representing additional figures, materials and methods explanation, or supporting results and evidence can be submitted with the main manuscript as supplementary material.