Extraction, purification, structural characterization, and anti-inflammatory activity of a polysaccharide from Lespedeza formosa.

Abstract

A new acidic polysaccharide was extracted from Lespedeza Formosa (LF) using microwave-assisted extraction. After a progressive purification, Lespedeza Formosa polysaccharide (LFP-1) with 96.14 % purity and moderate molecular weight was obtained. Subsequently, LFP-1's structural analysis and in vitro anti-inflammatory experiments were performed. LFP-1 is composed of nine monosaccharides, mainly including 39.7 % glucose, 29.1 % galactose, and 19.9 % arabinose, with three branched chains of its structure. The diverse monosaccharides and branched chains provided the essential conditions for the anti-inflammatory effects of LFP-1, which diminished the release of nitric oxide (NO) and reactive oxygen species (ROS). And they altered the release of internal inflammatory factors in lipopolysaccharide (LPS)-treated macrophages. LFP-1 exerted intracellularly anti-inflammatory effects through the nuclear factor kappa-B (NF-κB) signal pathway. The discovery of LFP-1 opens up a new possibility for natural anti-inflammatory medicine.