Electrochemical aptasensor for the selective detection of vancomycin based on nanostructured “in-lab” printed electrodes

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL Microchimica Acta Pub Date : 2025-01-25 DOI:10.1007/s00604-025-06952-1

Malek Bibani, Magdolna Casian, Bogdan Feier, Diana Bogdan, Oana Hosu-Stancioiu, Nadia Ktari, Rafik Kalfat, Cecilia Cristea

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Abstract

A label-free, flexible, and disposable aptasensor was designed for the rapid on-site detection of vancomycin (VAN) levels. The electrochemical sensor was based on lab-printed carbon electrodes (C-PE) enriched with cauliflower-shaped gold nanostructures (AuNSs), on which VAN-specific aptamers were immobilized as biorecognition elements and short-chain thiols as blocking agents. The AuNSs, characterized by scanning electron microscopy (SEM) and atomic force microscopy (AFM), enhanced the electrochemical properties of the platform and the aptamer immobilization active sites. The developed disposable aptasensor allowed label-free detection of VAN via electrochemical impedance spectroscopy (EIS) across a wide range of concentrations (50–1000 nM), with a limit of detection (LOD) of 1.721 nM. The aptasensor presented good selectivity against some commonly found interferences in human serum and milk and was successfully applied to the analysis of these samples.

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基于纳米结构 "实验室内 "印刷电极的选择性检测万古霉素的电化学传感器。

设计了一种无标签、灵活、一次性的万古霉素适体传感器，用于万古霉素的快速现场检测。该电化学传感器基于实验室打印的富含菜花形状金纳米结构的碳电极（C-PE），其上固定了van特异性适配体作为生物识别元件，短链硫醇作为阻断剂。通过扫描电镜（SEM）和原子力显微镜（AFM）对AuNSs进行了表征，发现其增强了平台的电化学性能和适配体的固定活性位点。开发的一次性适体传感器可以通过电化学阻抗谱（EIS）在很宽的浓度范围内（50-1000 nM）进行无标签检测，检测限（LOD）为1.721 nM。该配体传感器对人血清和牛奶中常见的干扰具有良好的选择性，并成功地应用于这些样品的分析。

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.