Transcriptomic Approaches to Investigate the Anti-Aging Effects of Blueberry Anthocyanins in a Caenorhabditis Elegans Aging Model.

IF 6.6 2区医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Antioxidants Pub Date : 2024-12-30 DOI:10.3390/antiox14010035

Jie Ding, Jiahui Liu, Qingqi Guo, Na Zhang

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Abstract

This study investigates the anti-aging effects of various concentrations of blueberry anthocyanins (BA) on the lifespan and health-related phenotypes of Caenorhabditis elegans. Blueberry anthocyanins were administered at concentrations of 50.0 μg/mL, 200.0 μg/mL, and 500.0 μg/mL, and their effects on nematode lifespan, locomotion, pharyngeal pumping rate, and the accumulation of lipofuscin and reactive oxygen species (ROS) were examined. Transcriptomic analysis was conducted to explore the regulatory effects of BA on anti-aging molecular pathways and key genes in C. elegans. Results showed a significant, dose-dependent extension of lifespan, improvement in locomotion and pharyngeal pumping rate, and reduction in lipofuscin and ROS accumulation. Transcriptomic analysis revealed that BA activated anti-aging pathways such as FOXO, IIS, and PI3K/Akt, upregulating critical genes like daf-16. These findings highlight the potential of blueberry anthocyanins as promising anti-aging agents through multiple physiological and molecular mechanisms.

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转录组学方法研究蓝莓花青素在秀丽隐杆线虫衰老模型中的抗衰老作用。

本研究探讨了不同浓度蓝莓花青素（BA）对秀丽隐杆线虫寿命和健康相关表型的抗衰老作用。分别以50.0、200.0和500.0 μg/mL浓度给药蓝莓花青素，观察其对线虫寿命、运动、咽泵速率、脂褐素和活性氧（ROS）积累的影响。通过转录组学分析，探讨BA对秀丽隐杆线虫抗衰老分子通路及关键基因的调控作用。结果显示显著的剂量依赖性寿命延长，运动和咽泵速率改善，脂褐素和ROS积累减少。转录组学分析显示，BA激活了FOXO、IIS和PI3K/Akt等抗衰老途径，上调了daf-16等关键基因。这些发现强调了蓝莓花青素通过多种生理和分子机制作为抗衰老药物的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Antioxidants Biochemistry, Genetics and Molecular Biology-Physiology

CiteScore

10.60

自引率

11.40%

发文量

2123

审稿时长

16.3 days

期刊介绍： Antioxidants (ISSN 2076-3921), provides an advanced forum for studies related to the science and technology of antioxidants. It publishes research papers, reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.