Rapid and sensitive detection of exosomal microRNAs by terahertz metamaterials

IF 4.6 2区化学 Q1 SPECTROSCOPY Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-04-05 Epub Date: 2025-01-16 DOI:10.1016/j.saa.2025.125745

Yi Zhou , Yu Liu , Zhen Zong , Honghao Huang , Ling Liang , Xiang Yang , Mei Xin , Huiyan Tian , Fengxin Xie , Weidong Jin , Jining Li , Ke Yang

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Abstract

Exosomal microRNAs (miRNAs) are among the most common biomarkers for tumor diagnosis. However, single-miRNA detection lacks ideal sensitivity and specificity for diagnosing a certain tumor in clinics. In this work, we fabricated a convenient multi-miRNA detection platform for sensitive and specific detection on exosomal miRNAs in the plasma of patients using a terahertz (THz) metamaterial biosensor on the basis of strand displacement amplification (SDA) and AuNPs. The proposed multi-miRNA detection platform was highly sensitive to miRNA-15, miRNA-21, miRNA-145, miRNA-155, miRNA-423, and miRNA-451, and the limit of detection (LOD) obtained were 12.54 aM, 19.66 aM, 17.50 aM, 25.40 aM, 24.11 aM and 26.59 aM, respectively. The biosensor we constructed can be used to diagnose pancreatic cancer patients effectively in complex clinical samples. The use of multiple miRNAs demonstrated strong practicality in the combined diagnosis of patients with pancreatic cancer. These studies demonstrate that the multi-RNA detection platform boasts advantages such as low cost, rapid, high sensitivity, and specificity, offering a potential tool for future clinical applications.

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用太赫兹超材料快速灵敏地检测外泌体microrna。

外泌体microRNAs （miRNAs）是肿瘤诊断中最常见的生物标志物之一。然而，单mirna检测在临床上对某一肿瘤的诊断缺乏理想的敏感性和特异性。在这项工作中，我们构建了一个方便的多mirna检测平台，用于基于链位移扩增（SDA）和AuNPs的太赫兹（THz）超材料生物传感器对患者血浆中外泌体mirna进行敏感和特异性检测。该多mirna检测平台对miRNA-15、miRNA-21、miRNA-145、miRNA-155、miRNA-423和miRNA-451高度敏感，获得的检出限（LOD）分别为12.54 aM、19.66 aM、17.50 aM、25.40 aM、24.11 aM和26.59 aM。我们构建的生物传感器可以在复杂的临床样本中有效地诊断胰腺癌患者。多种mirna的使用在胰腺癌患者的联合诊断中显示出很强的实用性。这些研究表明，该多rna检测平台具有成本低、快速、灵敏度高、特异性强等优点，为未来的临床应用提供了潜在的工具。

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.