Mechanism of the Discontinuous Structure in Heat-Induced Natural Egg Yolk Mediated by Accumulation of Yolk Sphere Microgels: Morphology, 4D-DIA Proteomics, and Physicochemical Properties

IF 5.7 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Journal of Agricultural and Food Chemistry Pub Date : 2025-01-27 DOI:10.1021/acs.jafc.4c11324

Keshan Wang, Xianmin Qin, Chan Li, Jiao Wu, Xiaoli Liu, Jiaxuan Ma, Ying Zhou, Qiujin Zhu, Yuanyuan Liu, Yongguo Jin

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Abstract

The heat-induced natural egg yolk is a discontinuous object formed by the accumulation of yolk spheres. However, the reason why yolk spheres form individual microgels rather than continuous gels has not been elucidated. This study investigated the different gelation behaviors in the yolk sphere exterior (EYSE) and the yolk sphere interior (EYSI) by using 4D-DIA proteomics, electron microscopy, and multispectral techniques. Results demonstrated that vitellogenin-1, -2, and -3 (EYSI/EYSE fold change: 3.36, 3.53, and 2.42, respectively) were key proteins corresponding to continuous gel structure formation of EYSI. However, the high levels of apolipoprotein A-I (FC: 0.18) and heat shock protein found in EYSE with a special hydrophobic domain for lipid binding impeded the continuous gel formation. Thus, the EYSE formed some small-volume aggregates without continuous gel, which separated individually the microgel of yolk spheres. This study will provide theoretical guidance for the quality regulation of egg yolk products.

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来源期刊

Journal of Agricultural and Food Chemistry 农林科学-农业综合

CiteScore

9.90

自引率

8.20%

发文量

1375

审稿时长

2.3 months

期刊介绍： The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.