Comparative pericarp biomechanics and germination physiology of Raphanus raphanistrum and Raphanus pugioniformis indehicent fruits.

Abstract

The biomechanical, morphological and ecophysiological properties of plant seed/fruit structures are adaptations that support survival in unpredictable environments. High phenotypic variability of noxious and invasive weed species such as Raphanus raphanistrum (wild radish) allow diversification into new environmental niches. Dry indehiscent fruits (thick and lignified pericarp [fruit coat] enclosing seeds) have evolved many times independently. Here, we demonstrate that the hard pericarp of Raphanus species (Brassicaceae) imposes mechanical dormancy by preventing full phase-II water uptake of the enclosed seeds. A multiscale biomechanics and imaging (microscopy, X-ray, finite element stress simulation, puncture force analysis) approach was used to comparatively investigate the indehiscent fruits of R. raphanistrum (global weed), R. pugioniformis (endemic weed) and R. sativus (cultivated radish). This demonstrated that the apparently unilocular fruits of Raphanus species develop from two fused valves, that pericarp rupture to permit germination is confined to the midvalve regions (MVR), and that each MVR contains a predeterimed breaking zone which is biomechanically defined by the internal shape of the seed chambers. Direct biomechanical analysis revealed great variability in within-fruit and between-fruits pericarp resistences. We conclude that variability in pericarp-imposed dormancy provides a bet-hedging strategy to affect soil seed bank persistence and prolong the germinability period.