Cytoplasmic streaming of symbiotic algae in the ciliate Stentor pyriformis.

Abstract

Stentor pyriformis is a unicellular organism whose inherent green-algal symbionts can be utilized in evolutionary and cytological studies. The cytoplasm contains symbiotic algae and starch granules, which are in constant motion. The habitats of the ciliate S. pyriformis are restricted to a few oligotrophic ponds in Japan. This study aimed to develop a culture medium for long-term incubation based on pond water quality data and to investigate the cytoplasmic streaming of symbiotic algae and starch granules in S. pyriformis. In addition, the involvement of the cytoskeleton and motor proteins in cytoplasmic streaming was examined using microtubule polymerization and dynein ATPase inhibitors. The results indicated that the cytoplasmic streaming in S. pyriformis is associated with the microtubule system. Immunofluorescence and transmission electron microscopy revealed the presence of KM-fibers, bundles of microtubules running longitudinally along the cell surface. These findings suggest a possible link between microtubules and cytoplasmic streaming in S. pyriformis.