Phosphotungstic acid-enhanced micro-computed tomography and RNA sequencing provide a new perspective on temporomandibular joint arthritis induced by complete Freund's adjuvant and collagen-induced arthritis in rat models.
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引用次数: 0
Abstract
Background/purpose: Temporomandibular joint (TMJ) arthritis causes inflammation and degradation of the mandibular condylar cartilage and subchondral bone. Complete Freund's adjuvant (CFA) and collagen-induced arthritis (CIA) are models for studying TMJ arthritis. While micro-computed tomography (micro-CT) is crucial for three-dimensional (3D) bone analysis, it has limitations in imaging nonmineralized tissues. Phosphotungstic acid (PTA) enhances soft tissue contrast. However, research on the 3D imaging of mandibular condylar cartilage and the molecular mechanisms of CFA- and CIA-induced arthritis remains unclear. This study aimed to investigate the bone and PTA-stained cartilage in the mandibular condyle using 3D reconstruction and explore the characteristics of enriched gene ontology terms underlying CFA- and CIA-induced TMJ arthritis in rat models.
Materials and methods: Rat mandibular condyles were collected from control, CFA, and CIA groups. Live micro-CT created 3D bone structures, and PTA-enhanced micro-CT constructed 3D mandibular condylar cartilage. Gene ontology enrichment analysis identified enriched gene ontology terms from differentially expressed genes through RNA sequencing.
Results: Major deformities in cartilage volume and bone morphology were observed in the arthritis-induced groups. The CIA group exhibited significant correlations between cartilage volume and bone parameters changes. Gene ontology enrichment analysis indicated fewer terms with upregulated differentially expressed genes related to inflammation and immune response in the CIA group than in the CFA group.
Conclusion: This study reveals distinct responses between CFA- and CIA-induced TMJ arthritis models. The CIA group exhibited strong correlations between cartilage volume and bone parameter changes and had less pronounced inflammation and immune response than the CFA group.
背景/目的：颞下颌关节（TMJ）关节炎会导致下颌骨髁状突软骨和软骨下骨的炎症和退化。完全弗氏佐剂（CFA）和胶原诱导关节炎（CIA）是研究颞下颌关节炎的模型。虽然显微计算机断层扫描（micro-CT）对三维（3D）骨分析至关重要，但它在非矿化组织成像方面存在局限性。磷钨酸（PTA）可增强软组织对比度。然而，下颌骨髁状突软骨的三维成像以及CFA和CIA诱发关节炎的分子机制研究仍不清楚。本研究旨在利用三维重建技术研究下颌骨髁状突软骨和PTA染色软骨，并探索大鼠模型中CFA和CIA诱导颞下颌关节炎的富集基因本体术语的特征：从对照组、CFA组和CIA组收集大鼠下颌骨髁突。活体显微 CT 绘制了三维骨结构，PTA 增强显微 CT 绘制了三维下颌骨髁状突软骨。基因本体富集分析通过 RNA 测序确定了差异表达基因的富集基因本体术语：结果：在关节炎诱发组中观察到软骨体积和骨形态的重大畸形。CIA组软骨体积和骨参数变化之间存在明显的相关性。基因本体富集分析表明，与 CFA 组相比，CIA 组中与炎症和免疫反应相关的差异表达基因上调的术语较少：本研究揭示了CFA和CIA诱导的颞下颌关节炎模型之间不同的反应。结论：本研究揭示了 CFA 和 CIA 诱导的颞下颌关节炎模型之间不同的反应，CIA 组软骨体积和骨参数变化之间具有很强的相关性，其炎症和免疫反应不如 CFA 组明显。
期刊介绍：
he Journal of Dental Sciences (JDS), published quarterly, is the official and open access publication of the Association for Dental Sciences of the Republic of China (ADS-ROC). The precedent journal of the JDS is the Chinese Dental Journal (CDJ) which had already been covered by MEDLINE in 1988. As the CDJ continued to prove its importance in the region, the ADS-ROC decided to move to the international community by publishing an English journal. Hence, the birth of the JDS in 2006. The JDS is indexed in the SCI Expanded since 2008. It is also indexed in Scopus, and EMCare, ScienceDirect, SIIC Data Bases.
The topics covered by the JDS include all fields of basic and clinical dentistry. Some manuscripts focusing on the study of certain endemic diseases such as dental caries and periodontal diseases in particular regions of any country as well as oral pre-cancers, oral cancers, and oral submucous fibrosis related to betel nut chewing habit are also considered for publication. Besides, the JDS also publishes articles about the efficacy of a new treatment modality on oral verrucous hyperplasia or early oral squamous cell carcinoma.
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