Recombinant nanobody expressed by transgenic chimeric chickens eliminated vertical transmission of avian leukosis virus subgroup J

IF 2.4 2区农林科学 Q3 MICROBIOLOGY Veterinary microbiology Pub Date : 2025-01-25 DOI:10.1016/j.vetmic.2025.110407

Jiahua Liang , Ke Qin , Ning Xiao , Yingying Zhao , Ruiying Han , Jinhan Wang , Jiahan Liu , Huicheng Lu , Xiaoli Wang , Fuxian Yu , Xiaochuan Tang

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Abstract

Because of the vertical transmission of avian leukosis virus subgroup J (ALV-J), control of ALV-J in breed of chicken is still a serious issue. Blocking vertical transmission using antibodies is a potential strategy, but its high cost limits its application. We artificially designed recombinant nanobody (Nb) and efficiently expressed and secreted them in three primary chicken cells cultured in vitro by adenovirus delivery. We found that secreted the Nb could reduce the infection of cells by ALV-J. Moreover, we prepared transgenic chimeric chickens expressing Nb and found that the Nb reduced the infection and vertical transmission of ALV-J to chickens. In particular, transgenic chimeric hens expressing high concentration of the Nb eliminated vertical transmission of the virus. Collectively, current study provides new avenues for the control of ALV-J transmission.

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由于禽白血病病毒 J 亚群（ALV-J）具有垂直传播性，因此在鸡种中控制 ALV-J 仍是一个严峻的问题。使用抗体阻断垂直传播是一种潜在的策略，但其高昂的成本限制了其应用。我们人工设计了重组纳米抗体（Nb），并通过腺病毒递送在体外培养的三个原代鸡细胞中高效表达和分泌。我们发现，分泌的 Nb 可减少 ALV-J 对细胞的感染。此外，我们还制备了表达 Nb 的转基因嵌合鸡，发现 Nb 可减少 ALV-J 对鸡的感染和垂直传播。特别是，表达高浓度 Nb 的转基因嵌合母鸡消除了病毒的垂直传播。总之，目前的研究为控制 ALV-J 的传播提供了新的途径。

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来源期刊

Veterinary microbiology 农林科学-兽医学

CiteScore

5.90

自引率

6.10%

发文量

221

审稿时长

52 days

期刊介绍： Veterinary Microbiology is concerned with microbial (bacterial, fungal, viral) diseases of domesticated vertebrate animals (livestock, companion animals, fur-bearing animals, game, poultry, fish) that supply food, other useful products or companionship. In addition, Microbial diseases of wild animals living in captivity, or as members of the feral fauna will also be considered if the infections are of interest because of their interrelation with humans (zoonoses) and/or domestic animals. Studies of antimicrobial resistance are also included, provided that the results represent a substantial advance in knowledge. Authors are strongly encouraged to read - prior to submission - the Editorials (''Scope or cope'' and ''Scope or cope II'') published previously in the journal. The Editors reserve the right to suggest submission to another journal for those papers which they feel would be more appropriate for consideration by that journal. Original research papers of high quality and novelty on aspects of control, host response, molecular biology, pathogenesis, prevention, and treatment of microbial diseases of animals are published. Papers dealing primarily with immunology, epidemiology, molecular biology and antiviral or microbial agents will only be considered if they demonstrate a clear impact on a disease. Papers focusing solely on diagnostic techniques (such as another PCR protocol or ELISA) will not be published - focus should be on a microorganism and not on a particular technique. Papers only reporting microbial sequences, transcriptomics data, or proteomics data will not be considered unless the results represent a substantial advance in knowledge. Drug trial papers will be considered if they have general application or significance. Papers on the identification of microorganisms will also be considered, but detailed taxonomic studies do not fall within the scope of the journal. Case reports will not be published, unless they have general application or contain novel aspects. Papers of geographically limited interest, which repeat what had been established elsewhere will not be considered. The readership of the journal is global.