Deciphering the mobility and bacterial hosts of antibiotic resistance genes under the coexistence of antibiotics and microplastics by metagenomic analysis and binning method
{"title":"Deciphering the mobility and bacterial hosts of antibiotic resistance genes under the coexistence of antibiotics and microplastics by metagenomic analysis and binning method","authors":"Ming Xu, Yuan Gao, Yun-xiang Zhu, Peng Gao, Heng Zhang, Xiao-xiao Shen","doi":"10.1016/j.cej.2025.160029","DOIUrl":null,"url":null,"abstract":"The effects of co-exposure to antibiotics and microplastics in waste-activated sludge on the spread of antibiotic resistance genes remain poorly understood. Metagenomic data obtained from anaerobic digestion reactors supplemented with tetracyclines and various microplastics were used to analyze the microbial community structure and functional potential. The coexistence of tetracyclines and microplastics promoted hydrolysis and enhanced the fermentation efficiency of anaerobic digestion for methane production. Tetracycline-Polyamide (13.34–1.45%) and Tetracycline-Polypropylene (13.34–10.05%) inhibited the removal efficiency of antibiotic resistance genes in anaerobic digestion, whereas Tetracycline-Polyethylene enhanced the removal efficiency of antibiotic resistance genes in this process (13.34–34.38%). The insertion sequences of mobile genetic elements significantly affected the dissemination of multidrug and aminoglycoside antibiotic resistance genes. The recovered metagenome-assembled genomes revealed a new antibiotic resistance gene host, the <em>Microtrichaceae</em> family, which exhibited multidrug resistance. Soluble proteins may serve as critical links between cellular metabolism and the propagation of antibiotic resistance genes within the tetracycline-microplastics system. Tetracycline-microplastics influenced antibiotic resistance gene transmission by regulating key genes associated with horizontal gene transfer, such as reactive oxygen species, cell membrane permeability, extracellular polymeric substance generation, and the type IV secretion system. This study provides novel insights into the transmission of antibiotic resistance genes exacerbated by combined pollution within the sludge digestion system and the mechanistic responses associated with horizontal gene transfer, thereby guiding the enhanced control of antibiotic resistance gene transmission.","PeriodicalId":270,"journal":{"name":"Chemical Engineering Journal","volume":"10 1","pages":""},"PeriodicalIF":13.3000,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemical Engineering Journal","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1016/j.cej.2025.160029","RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, CHEMICAL","Score":null,"Total":0}
引用次数: 0
Abstract
The effects of co-exposure to antibiotics and microplastics in waste-activated sludge on the spread of antibiotic resistance genes remain poorly understood. Metagenomic data obtained from anaerobic digestion reactors supplemented with tetracyclines and various microplastics were used to analyze the microbial community structure and functional potential. The coexistence of tetracyclines and microplastics promoted hydrolysis and enhanced the fermentation efficiency of anaerobic digestion for methane production. Tetracycline-Polyamide (13.34–1.45%) and Tetracycline-Polypropylene (13.34–10.05%) inhibited the removal efficiency of antibiotic resistance genes in anaerobic digestion, whereas Tetracycline-Polyethylene enhanced the removal efficiency of antibiotic resistance genes in this process (13.34–34.38%). The insertion sequences of mobile genetic elements significantly affected the dissemination of multidrug and aminoglycoside antibiotic resistance genes. The recovered metagenome-assembled genomes revealed a new antibiotic resistance gene host, the Microtrichaceae family, which exhibited multidrug resistance. Soluble proteins may serve as critical links between cellular metabolism and the propagation of antibiotic resistance genes within the tetracycline-microplastics system. Tetracycline-microplastics influenced antibiotic resistance gene transmission by regulating key genes associated with horizontal gene transfer, such as reactive oxygen species, cell membrane permeability, extracellular polymeric substance generation, and the type IV secretion system. This study provides novel insights into the transmission of antibiotic resistance genes exacerbated by combined pollution within the sludge digestion system and the mechanistic responses associated with horizontal gene transfer, thereby guiding the enhanced control of antibiotic resistance gene transmission.
期刊介绍：
The Chemical Engineering Journal is an international research journal that invites contributions of original and novel fundamental research. It aims to provide an international platform for presenting original fundamental research, interpretative reviews, and discussions on new developments in chemical engineering. The journal welcomes papers that describe novel theory and its practical application, as well as those that demonstrate the transfer of techniques from other disciplines. It also welcomes reports on carefully conducted experimental work that is soundly interpreted. The main focus of the journal is on original and rigorous research results that have broad significance. The Catalysis section within the Chemical Engineering Journal focuses specifically on Experimental and Theoretical studies in the fields of heterogeneous catalysis, molecular catalysis, and biocatalysis. These studies have industrial impact on various sectors such as chemicals, energy, materials, foods, healthcare, and environmental protection.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
