Hemoadhican Fiber Composite with Carbon Dots for Treating Severe Hemorrhage and Infected Wounds

Abstract

Uncontrolled bleeding and infection following trauma continue to pose significant clinical challenges. This study employs hemoadhican (HD) polysaccharide, known for its superior hemostatic properties, as the foundational material to synthesize antibacterial carbon dots (H-CDs) through a hydrothermal method at various temperatures. The H-CDs exhibiting optimal antimicrobial properties were identified via in vitro antimicrobial characterization. The selected H-CDs possess nanoscale dimensions and a positive surface charge. They contain aldehyde groups and generate reactive oxygen species, which effectively eliminate bacteria. Subsequently, H-CDs were integrated into HD fibers (CDs-HD fibers) using a wet-spinning technique. The water vapor transmission rate, blood contact angle, and in vitro antimicrobial efficacy were evaluated. In a rat model of severe femoral artery hemorrhage and a noncompressible hepatic hemorrhage model, CDs-HD fibers demonstrated superior hemostatic performance compared to the commercially available QuikClot Combat Gauze. Furthermore, in a rat model of mixed bacterial wound infection, CDs-HD fibers significantly enhanced epithelial tissue remodeling and collagen deposition. In vivo studies confirmed the excellent biocompatibility of CDs-HD fibers. These findings suggest that CDs-HD fibers hold promise as a potential dressing for managing severe bleeding and preventing wound infections.