IS15DIV-flanked composite transposon harboring blaNDM-5 in multidrug-resistant Salmonella Typhimurium

IF 4.6 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES iScience Pub Date : 2024-12-31 DOI:10.1016/j.isci.2024.111720

Kaiting Zhao , Jing Jin , Yuan Liao , Aixia Liu , Wugao Liu , Weiping Wu

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Abstract

Multidrug-resistant Salmonella Typhimurium has emerged as a global public health concern. Asymptomatic gastrointestinal carriage is a key factor in the spread of antibiotic-resistant bacteria. However, it is challenging to obtain direct evidence of in vivo transfer of mobile genetic elements (MGEs). Here, we found that MGEs harboring bla_NDM-5 were transferred from asymptomatic Escherichia coli (LS20223694) to Salmonella Typhimurium (LS20223695) in a child. BLAST analyses demonstrated that the IS15DIV-flanked composite transposon of pLS20223695_NDM5 showed high similarity with pLS20223694_NDM5 and two previously reported plasmids, suggesting the possibility of genetic recombination. Besides, conjugation experiments showed that the transconjugant carried the incompatibility group I1 (IncI1)-I(alpha) plasmid replicon with the bla_NDM-5 and sul1 genes, indicating that pLS20223695_NDM5 is a conjugative plasmid with transferability. Our study provides insights into the genetic basis of an IS15DIV-flanked composite transposon in Salmonella Typhimurium.

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来源期刊

iScience Multidisciplinary-Multidisciplinary

CiteScore

7.20

自引率

1.70%

发文量

1972

审稿时长

6 weeks

期刊介绍： Science has many big remaining questions. To address them, we will need to work collaboratively and across disciplines. The goal of iScience is to help fuel that type of interdisciplinary thinking. iScience is a new open-access journal from Cell Press that provides a platform for original research in the life, physical, and earth sciences. The primary criterion for publication in iScience is a significant contribution to a relevant field combined with robust results and underlying methodology. The advances appearing in iScience include both fundamental and applied investigations across this interdisciplinary range of topic areas. To support transparency in scientific investigation, we are happy to consider replication studies and papers that describe negative results. We know you want your work to be published quickly and to be widely visible within your community and beyond. With the strong international reputation of Cell Press behind it, publication in iScience will help your work garner the attention and recognition it merits. Like all Cell Press journals, iScience prioritizes rapid publication. Our editorial team pays special attention to high-quality author service and to efficient, clear-cut decisions based on the information available within the manuscript. iScience taps into the expertise across Cell Press journals and selected partners to inform our editorial decisions and help publish your science in a timely and seamless way.