Rhodamine-functionalized carbon dots with pH-regulated FRET efficiency for ratiometric fluorescence sensing and imaging of extremely alkaline pH

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL Microchimica Acta Pub Date : 2025-01-28 DOI:10.1007/s00604-024-06941-w

Jing-Yuan Zhang, Dan Wang, Jia Li, Ying Tian, Si Hui Yang

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Abstract

A ratiometric fluorescent nanoprobe (CDs-Rho), synthesized through the simple covalent amide linkage between carbon dots (CDs) and pH-sensitive rhodamine dye (Rho), was designed for the precise sensing and imaging of extremely alkaline environments. The sensing mechanism involves the opposite pH-dependent fluorescence changes in CDs and Rho, respectively, coupled with pH-regulated FRET efficiency from CDs to Rho. The nanoprobe features a wide pH response window from pH 7.0 to 12.0 with a pK_a value of 11.3 and shows high sensitivity, robust anti-interference capability, and high reversibility. Moreover, the significant shifts in emission wavelength following the pH fluctuations result in two well-separated emission signals, thus ensuring the visualization of reversible and distinct color changes (from green to red) during in vivo fluorescence imaging. This work furnished a facile protocol that contributes to the advancement of a novel method for the accurate sensing and imaging of extreme alkaline environments.

Graphical abstract

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具有pH调节FRET效率的罗丹明功能化碳点用于极碱性pH的比例荧光传感和成像。

通过碳点（CDs）与ph敏感罗丹明染料（Rho）之间的简单共价酰胺键合成了一种比率荧光纳米探针（CDs-Rho），用于对极碱性环境的精确传感和成像。感应机制涉及CDs和Rho中相反的ph依赖性荧光变化，以及从CDs到Rho的ph调节FRET效率。该纳米探针具有pH 7.0 ~ 12.0的宽响应窗口，pKa值为11.3，灵敏度高，抗干扰能力强，可逆性强等特点。此外，随着pH值的波动，发射波长的显著变化导致了两个分离良好的发射信号，从而确保了在体内荧光成像中可见可逆且明显的颜色变化（从绿色到红色）。这项工作提供了一个简单的协议，有助于推进一种新的方法，用于极端碱性环境的精确传感和成像。

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.