Flower-like Biomimetic Enzyme for Rapid and Sensitive Detection of Zearalenone in Vegetable Oil Deodorizer Distillate.

Abstract

In order to achieve high quality production of vitamin E and plant sterols, it is necessary to conduct rapid and accurate detection of fungal toxins in their production raw material (vegetable oil deodorizer distillate, VODD). In this study, the flower-like biomimetic enzyme of silver-doped ZnO was synthesized through wet chemical method and in-situ reduction method. Based on above work, a flower-like biomimetic enzyme modified glass carbon electrode was fabricated, and its excellent detection capability against fungal toxins zearalenone was confirmed through electrochemical analysis. The detection limit was 8 ng mL^-1, with a linear range of 40 ng mL^-1-25 μg mL^-1. Simultaneously, the biomimetic enzyme sensor takes only 10 minutes from preparation to completion of detection, and the RSD between the 7 repeated test results was only 0.612 %. After seven days of storage, the current response value remains 91.5 % of the initial value. In practical applications, the recovery rate of zearalenone in VODD using this sensor ranged from 98.1% to 102.08 %, yielding satisfactory results. Therefore, the novel flower-like biomimetic enzyme represents an ideal choice for developing zearalenone sensors and holds promising prospects for wide application in fungal toxins analysis.