Response of an obligate CAM plant to competition and increased watering intervals.

Abstract

Climate change has exacerbated precipitation variability, profoundly impacting vegetation dynamics and community structures in arid ecosystems. There remains a notable knowledge gap regarding the ecological effects of altered precipitation on crassulacean acid metabolism (CAM) plants and their interactions with other photosynthetic types. This study investigated the response of the typical obligate CAM plant Orostachys fimbriata to extended watering intervals (WI4-WI8) and various competitive patterns (M₁-M₄) with the C₃ grass Melilotus officinalis and the C₄ grass Setaria viridis through greenhouse experiments. The results showed that: (1) In species mixtures, CAM plants had slightly reduced the total biomass (TB) compared to monocultures, yet maintained competitiveness by increasing the root-to-shoot biomass (R:S) ratio, stabilizing plant height, and sustaining their photosynthetic rates. (2) As watering intervals increased, CAM plants adapted by further elevating the R:S ratio, reducing height, and decreasing aboveground biomass. However, their height, CO₂ assimilation rate, and above- and below-ground biomass were significantly suppressed, particularly when coexisting with C₄ plants. More extreme watering regime caused a 47.6% decrease in TB of CAM plants in M₄, while C₃ and C₄ grasses declined by 53.2% and 37.8%, respectively. (3) Given the predicted extension of drought intervals and the intensification of individual rainfall events under future climate conditions, the competitive pressure from C₄ plants with high drought tolerance and resource acquisition advantages may limit the expansion potential of CAM plants in drylands. This study enhances the understanding of adaptive mechanisms of CAM plants competing and coexisting with grasses under variable environments, providing scientific bases for predicting arid ecosystem dynamics.