Arabidopsis Ankyrin-Repeat Protein Kinase ANK-PK2 Negatively Regulates Salt Tolerance by Mediating Degradation of the Sugar Transporter Protein STP11.

Abstract

Soluble sugars provide energy sources required for plant growth and development. They also act as osmoprotectants to improve the salt tolerance of plants. However, molecular mechanism underlying the negative regulation of soluble sugar accumulation in plants under salt stress conditions remains unknown. In this study, we investigated the functions of ankyrin-repeat kinase 2 (ANK-PK2) that regulates soluble sugar content in Arabidopsis under salt stress. ANK-PK2 interacts with and phosphorylates the sugar transporter protein 11 (STP11) under salt stress. Phosphorylated STP11 is easier to degrade, and its glucose-transporting ability and soluble sugar accumulation are inhibited. The ank-pk2 mutant exhibited increased salt tolerance. The salt-sensitive phenotype of the mutant stp11 was recovered through a dephosphorylation mutation that changed Thr²²⁷ in STP11 to Ala²²⁷. Our results revealed a novel molecular mechanism underlying salt stress adaptation in Arabidopsis, which ANK-PK2 negatively regulates salt tolerance by phosphorylating and subsequently decreasing the transport activity of STP11 to balance the cellular soluble sugar content in Arabidopsis.