Pretreatment of enzymatic hydrolysis lignin based on deep eutectic solvent containing a reversibly-soluble base.

IF 8.5 1区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY International Journal of Biological Macromolecules Pub Date : 2025-04-01 Epub Date: 2025-01-28 DOI:10.1016/j.ijbiomac.2025.140452

Hui-Jun Liu, Da-Yu Sun, Lei Yang, Guang-Hui Ma, Rong-Ying Xia, Zi-Qi Wang, Ming-Zhu Yao, Li-Jing Gao, Rui-Ping Wei, Xiao-Mei Pan, Guo-Min Xiao

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Abstract

The pretreatment with green deep eutectic solvents (DESs) is conducive to realizing the high-efficiency utilization of lignin at a low cost. In this study, an innovative choline chloride/urea/calcium hydroxide (ChCl/UR/Ca(OH)₂) DES containing a reversibly-soluble base Ca(OH)₂ was developed for the pretreatment of enzymatic hydrolysis lignin (EHL). The lignin pretreatment effects of the proposed ChCl/UR/Ca(OH)₂ DES were compare with a series of DESs. The results indicated that ChCl/UR/20 % Ca(OH)₂ with a reversibly-soluble base addition of 20 % was permitted to achieve the optimal recovery yield of 81.067 % under mild conditions (80 °C, 7 h). The physical and chemical structure, molecular weight, thermal stability, content of phenolic hydroxyl and carboxyl groups, and recycling performance of DES were compared between ChCl/UR-L and ChCl/UR/20 % Ca(OH)₂-L. The results showed that ChCl/UR/20 % Ca(OH)₂-L retained the lignin skeleton structure and possessed preferable thermal stability and antioxidant activity, which was mainly attributed to the homogeneous molecular structure and abundant phenolic hydroxyl group contents. ChCl/UR/20 % Ca(OH)₂ was more advantageous for recycling, maintaining a lignin recovery yield of 57.400 % after 5 cycles, which was higher than that of most acidic and neutral DESs for regenerating lignin. This work provides a valuable reference on the valorization of lignin by using renewable DES.

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含可逆可溶碱的深共晶溶剂对酶解木质素的预处理。

绿色深共晶溶剂预处理有利于以低成本实现木质素的高效利用。本研究开发了一种新型的氯化胆碱/尿素/氢氧化钙（ChCl/UR/Ca(OH)2） DES，用于酶解木质素（EHL）的预处理。比较了ChCl/UR/Ca(OH)2 DES对木质素的预处理效果。结果表明，在温和条件下（80 °C, 7 h）， ChCl/UR/20 % Ca(OH)2，可逆碱添加量为20 %时，回收率可达81.067 %。比较了ChCl/UR- l和ChCl/UR/20 % Ca(OH)2-L的DES的理化结构、分子量、热稳定性、酚羟基和羧基含量以及回收性能。结果表明：ChCl/UR/20 % Ca(OH)2-L保留了木质素骨架结构，具有较好的热稳定性和抗氧化活性，这主要归功于其分子结构均匀，酚羟基含量丰富。ChCl/UR/20 % Ca(OH)2更有利于再生，在5次 循环后，木质素回收率保持在57.400 %，高于大多数酸性和中性DESs再生木质素。本研究为利用可再生DES催化木质素增值提供了有价值的参考。

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文献相关原料

公司名称

产品信息

麦克林

Choline chloride

阿拉丁

Acetyl bromide

阿拉丁

P-hydroxybenzoic acid

来源期刊

International Journal of Biological Macromolecules 生物-生化与分子生物学

CiteScore

13.70

自引率

9.80%

发文量

2728

审稿时长

64 days

期刊介绍： The International Journal of Biological Macromolecules is a well-established international journal dedicated to research on the chemical and biological aspects of natural macromolecules. Focusing on proteins, macromolecular carbohydrates, glycoproteins, proteoglycans, lignins, biological poly-acids, and nucleic acids, the journal presents the latest findings in molecular structure, properties, biological activities, interactions, modifications, and functional properties. Papers must offer new and novel insights, encompassing related model systems, structural conformational studies, theoretical developments, and analytical techniques. Each paper is required to primarily focus on at least one named biological macromolecule, reflected in the title, abstract, and text.