Chemical shift assignments of DRB2 domains, a dsRNA binding protein in A. thaliana RNAi pathway

Abstract

In Arabidopsis thaliana, micro-RNA regulation is primarily controlled by DCL1, an RNase III enzyme, and its associated proteins. DCL1, together with DRB2, governs a specific group of miRNAs that induce the inhibition of target mRNA translation. DRB2 is a multi-domain protein containing two N-terminal dsRNA binding domains (dsRBD) separated by a linker, followed by an unstructured C-terminal tail. The two dsRBDs in DRB2 are involved in recognizing the miRNA precursor and aiding DCL1 in generating 21-nucleotide-long miRNA. Our study presents a nearly complete backbone chemical shift assignment of both dsRBDs and the side-chain assignment of the first dsRBD in DRB2. The data presented here lays the groundwork for future investigations into the structural, dynamic, and functional aspects of DRB2.