Bacillus pumilus G5 combined with silicon enhanced flavonoid biosynthesis in drought-stressed Glycyrrhiza uralensis Fisch. by regulating jasmonate, gibberellin and ethylene crosstalk

IF 5.7 2区生物学 Q1 PLANT SCIENCES Plant Physiology and Biochemistry Pub Date : 2025-03-01 Epub Date: 2025-01-27 DOI:10.1016/j.plaphy.2025.109560

Yonggan Ji , Duoyong Lang , Zhanchao Xu , Xin Ma , Qiuxian Bai , Wenjin Zhang , Xinhui Zhang , Qipeng Zhao

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Abstract

Drought stress poses a significant threat to global agricultural production, including the cultivation of medicinal plants. Plant growth-promoting bacteria (PGPB) and the eco-friendly element silicon (Si) are known to alleviate the adverse effects of drought stress. This study examines how inoculation with Bacillus pumilus G5 or/and Si influences plant hormone signaling and flavonoid biosynthesis pathways in drought-stressed Glycyrrhiza uralensis Fisch. (G. uralensis), focusing on genetic and metabolic aspects. The results indicate that the combined application of G5 and Si (G5+Si) may regulate the crosstalk among jasmonate (JA), gibberellin (GA), and ethylene (ET) signaling pathways, thereby up-regulating key flavonoid biosynthesis genes, including phenylalanine ammonia-lyase (PAL), cinnamate 4-hydroxylase (C4H), 4-coumarate-CoA ligase (4CL), and chalcone synthase (CHS), leading to the accumulation of isoliquiritigenin, liquiritigenin, liquiritin, and licochalcone A, thereby enhancing the drought tolerance of G. uralensis seedlings. The findings provide new insights into the synergistic role of PGPB and Si in improving plant resilience to drought stress, offering theoretical reference for further studies on plant drought tolerance mechanisms.

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枯草芽孢杆菌G5与硅联合促进干旱胁迫甘草类黄酮生物合成。通过调节茉莉酸、赤霉素和乙烯串扰。

干旱胁迫对全球农业生产构成重大威胁，包括药用植物的种植。植物生长促进菌（PGPB）和生态友好元素硅（Si）可以缓解干旱胁迫的不利影响。本研究探讨了接种短小芽孢杆菌G5或/和Si对干旱胁迫甘草激素信号传导和黄酮类生物合成途径的影响。（G. uralensis），关注遗传和代谢方面。结果表明，G5和Si （G5+Si）配施可调节茉莉酸（JA）、赤霉素（GA）和乙烯（ET）信号通路间的串音，从而上调苯丙氨酸解氨酶（PAL）、肉桂酸4-羟化酶（C4H）、4-香豆酸-辅酶A连接酶（4CL）和查尔酮合成酶（CHS）等关键类黄酮生物合成基因，导致异甘草素、甘草素、甘草素和甘草查尔酮A的积累。从而提高乌拉尔松幼苗的耐旱性。该研究结果为PGPB和Si在提高植物抗旱能力中的协同作用提供了新的认识，为进一步研究植物抗旱机制提供了理论参考。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.