Bacillus pumilus G5 combined with silicon enhanced flavonoid biosynthesis in drought-stressed Glycyrrhiza uralensis Fisch. by regulating jasmonate, gibberellin and ethylene crosstalk.

IF 6.1 2区生物学 Q1 PLANT SCIENCES Plant Physiology and Biochemistry Pub Date : 2025-01-27 DOI:10.1016/j.plaphy.2025.109560

Yonggan Ji, Duoyong Lang, Zhanchao Xu, Xin Ma, Qiuxian Bai, Wenjin Zhang, Xinhui Zhang, Qipeng Zhao

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引用次数: 0

Abstract

Drought stress poses a significant threat to global agricultural production, including the cultivation of medicinal plants. Plant growth-promoting bacteria (PGPB) and the eco-friendly element silicon (Si) are known to alleviate the adverse effects of drought stress. This study examines how inoculation with Bacillus pumilus G5 or/and Si influences plant hormone signaling and flavonoid biosynthesis pathways in drought-stressed Glycyrrhiza uralensis Fisch. (G. uralensis), focusing on genetic and metabolic aspects. The results indicate that the combined application of G5 and Si (G5+Si) may regulate the crosstalk among jasmonate (JA), gibberellin (GA), and ethylene (ET) signaling pathways, thereby up-regulating key flavonoid biosynthesis genes, including phenylalanine ammonia-lyase (PAL), cinnamate 4-hydroxylase (C4H), 4-coumarate-CoA ligase (4CL), and chalcone synthase (CHS), leading to the accumulation of isoliquiritigenin, liquiritigenin, liquiritin, and licochalcone A, thereby enhancing the drought tolerance of G. uralensis seedlings. The findings provide new insights into the synergistic role of PGPB and Si in improving plant resilience to drought stress, offering theoretical reference for further studies on plant drought tolerance mechanisms.

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.