Identification, molecular characterization and expression patterns of Cathepsin L in Yesso scallop (Patinopecten yessoensis) shell-infested by Polydora

Abstract

Patinopecten yessoensis (Yesso scallop), one of the most important aquaculture molluscs in China, has recently suffered severe Polydora disease, causing economic losses. Cathepsin L (CatL), a cysteine protease, has important functions in immune responses in vertebrates and invertebrates. However, little is known regarding the structure and function of CatL in scallops. In this study, a CatL gene named PyCatL was first identified in the genome of P. yessoensis. Gene structure analysis of PyCatL revealed it had 8 exons and 7 introns and a full length of 7916 bp. The gene sequence was analysed, and typically conserved functional domains (signal peptide, inhibitor I29 domain, and peptidase C1 domain) and motifs (ERWNIN, GNYD and GCXGG) of CatL were all predicted in PyCatL, confirming the sequence as belonging to a CatL gene. Phylogenetic analysis showed the evolutionary status of CatL was consistent with the species taxonomy. PyCatL was expressed ubiquitously in all the tested tissues in this study, suggesting its involvement in a wide range of physiological processes. After Polydora infestation, PyCatL exhibited significant upregulation in various mantle regions at both mRNA and protein levels, contrasting with a notable decrease in gene expression in hemocytes. Additionally, the enzyme activity of PyCatL showed a significant increase in the mantle of diseased P. yessoensis. The results suggested a role for mantle tissue in response to Polydora infestation by upregulating expression of PyCatL. The study offers novel insights into the function of CatL in innate immunity in scallops.