Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology最新文献

Effect of prebiotics added to the culture water on the bacterial profiles of biofloc and the gills, hepatopancreas, and intestine of Penaeus vannamei

IF 1.9 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-04-01 DOI: 10.1016/j.cbpb.2025.111097

Misael Rosales-Leija , Delbert M. Gatlin III , Jonathan P. Holt , Addison L. Lawrence

Prebiotics alter microbial communities by supplying carbon to specific bacteria that benefit the host. However, the effect of prebiotics added to the culture water on shrimp production and bacterial composition has not been demonstrated. Therefore, this study evaluated the effects of four prebiotics — short-chain fructooligosaccharide, galactooligosaccharide, inulin, and mannan-oligosaccharide — and two non-prebiotic carbohydrates — sucrose and wheat starch — on shrimp growth, nutrient composition of shrimp muscle and biofloc, biofloc concentration, and bacterial profiles in biofloc and gills, hepatopancreas, and intestine of Penaeus vannamei. Shrimp (6.0 ± 0.5 g) were stocked into 36 tanks (72 shrimp/m²) and raised in a biofloc technology system consisting of two phases: an autotrophic-dominated phase (days 0–4) and a heterotrophic-dominated phase (days 5–26). The prebiotic and non-prebiotic carbohydrates were added to the water at a rate of 3 % of the feed provided. Key findings revealed that adding prebiotics to the water did not affect shrimp growth. Notably, shrimp muscle from the mannan-oligosaccharide treatment exhibited a significantly higher lipid content than that from the galactooligosaccharide treatment (p ≤ 0.05). No significant effect was observed on the nutrient composition of biofloc. Biofloc concentration was lower in the wheat starch treatment (p ≤ 0.05). Finally, the prebiotics altered the bacterial composition of biofloc and shrimp gills, hepatopancreas, and intestine. These results revealed that adding prebiotics to the culture water of a biofloc system has an effect on shrimp quality and microbial communities of biofloc and various shrimp tissues, suggesting their potential benefit on shrimp production and health.

益生素通过向特定细菌提供碳来改变微生物群落，从而使宿主受益。然而，添加到养殖水中的益生元对对虾产量和细菌组成的影响尚未得到证实。因此，本研究评估了四种益生元（短链果寡糖、半乳寡糖、菊粉和甘露寡糖）和两种非益生元碳水化合物（蔗糖和小麦淀粉）对对虾生长、对虾肌肉和生物絮团的营养成分、生物絮团浓度以及生物絮团、对虾鳃、肝胰脏和肠道中细菌特征的影响。将对虾（6.0 ± 0.5 克）放入 36 个水箱（72 只/平方米），在生物絮凝技术系统中养殖，该系统包括两个阶段：自养为主的阶段（第 0-4 天）和异养为主的阶段（第 5-26 天）。益生菌和非益生碳水化合物按所提供饲料的 3% 的比例添加到水中。主要研究结果表明，在水中添加益生素不会影响虾的生长。值得注意的是，来自甘露寡糖处理的对虾肌肉的脂质含量明显高于来自半乳寡糖处理的对虾肌肉的脂质含量（p ≤ 0.05）。生物絮凝物的营养成分没有受到明显影响。小麦淀粉处理的生物絮凝物浓度较低（p ≤ 0.05）。最后，益生素改变了生物絮凝物和对虾鳃、肝胰腺和肠道的细菌组成。这些结果表明，在生物絮团系统的养殖水中添加益生素对对虾的质量以及生物絮团和对虾各种组织的微生物群落有影响，表明益生素对对虾的生产和健康有潜在的益处。

{"title":"Effect of prebiotics added to the culture water on the bacterial profiles of biofloc and the gills, hepatopancreas, and intestine of Penaeus vannamei","authors":"Misael Rosales-Leija , Delbert M. Gatlin III , Jonathan P. Holt , Addison L. Lawrence","doi":"10.1016/j.cbpb.2025.111097","DOIUrl":"10.1016/j.cbpb.2025.111097","url":null,"abstract":"<div><div>Prebiotics alter microbial communities by supplying carbon to specific bacteria that benefit the host. However, the effect of prebiotics added to the culture water on shrimp production and bacterial composition has not been demonstrated. Therefore, this study evaluated the effects of four prebiotics — short-chain fructooligosaccharide, galactooligosaccharide, inulin, and mannan-oligosaccharide — and two non-prebiotic carbohydrates — sucrose and wheat starch — on shrimp growth, nutrient composition of shrimp muscle and biofloc, biofloc concentration, and bacterial profiles in biofloc and gills, hepatopancreas, and intestine of <em>Penaeus vannamei</em>. Shrimp (6.0 ± 0.5 g) were stocked into 36 tanks (72 shrimp/m<sup>2</sup>) and raised in a biofloc technology system consisting of two phases: an autotrophic-dominated phase (days 0–4) and a heterotrophic-dominated phase (days 5–26). The prebiotic and non-prebiotic carbohydrates were added to the water at a rate of 3 % of the feed provided. Key findings revealed that adding prebiotics to the water did not affect shrimp growth. Notably, shrimp muscle from the mannan-oligosaccharide treatment exhibited a significantly higher lipid content than that from the galactooligosaccharide treatment (<em>p</em> ≤ 0.05). No significant effect was observed on the nutrient composition of biofloc. Biofloc concentration was lower in the wheat starch treatment (p ≤ 0.05). Finally, the prebiotics altered the bacterial composition of biofloc and shrimp gills, hepatopancreas, and intestine. These results revealed that adding prebiotics to the culture water of a biofloc system has an effect on shrimp quality and microbial communities of biofloc and various shrimp tissues, suggesting their potential benefit on shrimp production and health.</div></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":"278 ","pages":"Article 111097"},"PeriodicalIF":1.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143782030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dietary short-chain fatty acid supplementation does not affect growth performance, metabolism, and oxidative status of European seabass (Dicentrarchus labrax) juveniles

IF 1.9 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-03-27 DOI: 10.1016/j.cbpb.2025.111096

F. Fontinha , N. Martins , R. Magalhães , H. Peres , A. Oliva-Teles

This study investigated the effects of dietary short-chain fatty acid (SCFA) supplementation, on growth performance, metabolism, and antioxidant status of European seabass juveniles. Six isoproteic (43 % crude protein) and isolipidic (18 % crude lipid) diets were formulated to include 0.25 and 0.50 % Sodium acetate (SA), Sodium propionate (SP), or Sodium butyrate (SB). A diet without SCFA supplementation was used as a control. The diets were fed to triplicate groups of European seabass juveniles (initial body weight of 15 g) for 56 days. The supplementation of SCFA in the diet had no impact on the growth, feed utilization, or body composition of seabass. In the intestine, gene expression of pyruvate kinase (pk) and glucokinase (gk), phosphoenolpyruvate carboxykinase (pepck), glucose facilitative carrier type 2 (glut2), and citrate synthase (cs) was lower in fish fed the SP0.50 diet than in the other groups. Moreover, fatty acid synthase (fas) gene expression was lower in fish fed the SA0.25, SA0.50, and SB0.25 diets than in the other groups. Further, catalase (CAT) and glutathione reductase (GR) activity and lipid peroxidation (LPO) levels showed no differences between groups. In contrast, glutathione peroxidase (GPX) activity was higher in fish fed the SP0.50 diet. In the liver, GR activity and LPO levels showed no differences between groups. In contrast, CAT activity was lower in all dietary treatments than in control, and GPX and G6PDH activity was lower in fish fed with the SB (0.25 and 0.50 %) diet than in the other diets. Overall, SCFA supplementation did not affect growth performance and feed utilization and only had minor effects on metabolism and antioxidant defense mechanisms.

{"title":"Dietary short-chain fatty acid supplementation does not affect growth performance, metabolism, and oxidative status of European seabass (Dicentrarchus labrax) juveniles","authors":"F. Fontinha , N. Martins , R. Magalhães , H. Peres , A. Oliva-Teles","doi":"10.1016/j.cbpb.2025.111096","DOIUrl":"10.1016/j.cbpb.2025.111096","url":null,"abstract":"<div><div>This study investigated the effects of dietary short-chain fatty acid (SCFA) supplementation, on growth performance, metabolism, and antioxidant status of European seabass juveniles. Six isoproteic (43 % crude protein) and isolipidic (18 % crude lipid) diets were formulated to include 0.25 and 0.50 % Sodium acetate (SA), Sodium propionate (SP), or Sodium butyrate (SB). A diet without SCFA supplementation was used as a control. The diets were fed to triplicate groups of European seabass juveniles (initial body weight of 15 g) for 56 days. The supplementation of SCFA in the diet had no impact on the growth, feed utilization, or body composition of seabass. In the intestine, gene expression of pyruvate kinase (<em>pk</em>) and glucokinase (<em>gk</em>), phosphoenolpyruvate carboxykinase (<em>pepck</em>), glucose facilitative carrier type 2 (<em>glut2</em>), and citrate synthase (<em>cs</em>) was lower in fish fed the SP0.50 diet than in the other groups. Moreover, fatty acid synthase (<em>fas</em>) gene expression was lower in fish fed the SA0.25, SA0.50, and SB0.25 diets than in the other groups. Further, catalase (CAT) and glutathione reductase (GR) activity and lipid peroxidation (LPO) levels showed no differences between groups. In contrast, glutathione peroxidase (GPX) activity was higher in fish fed the SP0.50 diet. In the liver, GR activity and LPO levels showed no differences between groups. In contrast, CAT activity was lower in all dietary treatments than in control, and GPX and G6PDH activity was lower in fish fed with the SB (0.25 and 0.50 %) diet than in the other diets. Overall, SCFA supplementation did not affect growth performance and feed utilization and only had minor effects on metabolism and antioxidant defense mechanisms.</div></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":"278 ","pages":"Article 111096"},"PeriodicalIF":1.9,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143744489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of acute low-temperature stress on respiratory metabolism, antioxidants, and metabolomics of red swamp crayfish, Procambarus clarkii

IF 1.9 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-03-25 DOI: 10.1016/j.cbpb.2025.111095

Yu Ding , Wenbin Sha , Yunfei Sun , Yongxu Cheng

Crayfish (Procambarus clarkii) aquaculture is threatened by abrupt temperature decreases caused by climatic phenomena, such as cold waves and seasonal fluctuations. In this study, crayfish were exposed to an abrupt temperature change from 17 °C to 7 °C for 24 h to investigate the effects of acute low-temperatures on respiratory metabolism, antioxidants, and metabolomics. The results showed that acute low-temperatures significantly reduced the activities of pyruvate kinase, lactate dehydrogenase, and succinate dehydrogenase in the gills and hemolymph, associated with decreases in anaerobic and aerobic respiratory capacities, and significant decreases in oxygen consumption, ammonia excretion, and maximum metabolic rates. Antioxidant enzymes in the hepatopancreas and hemolymph initially increased then decreased within 24 h. Metabolomics revealed that glycerophospholipid metabolism and glycosylphosphatidylinositol anchor biosynthesis pathways responded to acute low-temperatures, with glycerophospholipids being the most significantly differentially expressed metabolites. These results supported the hypothesis that crayfish exhibit lower metabolic activity at low temperatures. Our data provide mechanistic insight into the biological changes induced by acute low-temperature and may provide insight into culture of P. clarkii in cold waters.

{"title":"Effects of acute low-temperature stress on respiratory metabolism, antioxidants, and metabolomics of red swamp crayfish, Procambarus clarkii","authors":"Yu Ding , Wenbin Sha , Yunfei Sun , Yongxu Cheng","doi":"10.1016/j.cbpb.2025.111095","DOIUrl":"10.1016/j.cbpb.2025.111095","url":null,"abstract":"<div><div>Crayfish (<em>Procambarus clarkii</em>) aquaculture is threatened by abrupt temperature decreases caused by climatic phenomena, such as cold waves and seasonal fluctuations. In this study, crayfish were exposed to an abrupt temperature change from 17 °C to 7 °C for 24 h to investigate the effects of acute low-temperatures on respiratory metabolism, antioxidants, and metabolomics. The results showed that acute low-temperatures significantly reduced the activities of pyruvate kinase, lactate dehydrogenase, and succinate dehydrogenase in the gills and hemolymph, associated with decreases in anaerobic and aerobic respiratory capacities, and significant decreases in oxygen consumption, ammonia excretion, and maximum metabolic rates. Antioxidant enzymes in the hepatopancreas and hemolymph initially increased then decreased within 24 h. Metabolomics revealed that glycerophospholipid metabolism and glycosylphosphatidylinositol anchor biosynthesis pathways responded to acute low-temperatures, with glycerophospholipids being the most significantly differentially expressed metabolites. These results supported the hypothesis that crayfish exhibit lower metabolic activity at low temperatures. Our data provide mechanistic insight into the biological changes induced by acute low-temperature and may provide insight into culture of <em>P. clarkii</em> in cold waters.</div></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":"278 ","pages":"Article 111095"},"PeriodicalIF":1.9,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143724471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insight into Ca2+- inositol 1,4,5-trisphosphate receptor 2 (IP3R2)-mediated unfolded protein response and apoptosis in scallop Patinopecten yessoensis under high temperature stress

IF 1.9 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-03-25 DOI: 10.1016/j.cbpb.2025.111092

Wenfei Gu , Xiaoxue Ma , Chuanyan Yang , Dongli Jiang , Hongmei Fan , Lingling Wang , Linsheng Song

Inositol 1,4,5-trisphosphate receptor 2 (IP₃R2) is an essential Ca²⁺ release channel protein located in the endoplasmic reticulum (ER), and plays a significant role in responding to various environmental stimuli. In the present study, the function of IP₃R2 from Yesso scallop Patinopecten yessoensis (PyIP₃R2) in regulating the Ca²⁺-mediated unfolded protein response (UPR) and apoptosis after high temperature (25 °C) treatment was investigated. Three MIR domains, one RYDR_ITPR domain, one RIH_assoc domain and one Ion_trans domain were identified in PyIP₃R2. Both D-myo-inositol-1,4,5-triphosphate (IP3, an activator of IP₃R) and high temperature significantly upregulated the mRNA expression level of PyIP₃R2 and genes related to apoptosis and the UPR, and also increased intracellular Ca²⁺ content (p < 0.05). Furthermore, the IP₃R antagonist 2-aminoethyl diphenylborinate (2-APB) had the opposite effect, decreasing intracellular Ca²⁺ content and the mRNA expression level of PyIP₃R2, glucose regulated protein 78 (PyGRP78) and PyCaspase-3 (p < 0.05). However, the apoptosis rate and Caspase-3 activity remained comparable to those in the injection control group. These findings indicate that PyIP₃R2 mediates UPR and apoptosis in scallop haemocytes by regulating Ca²⁺content and distribution, and providing insight into the cellular responses of scallops to high temperature.

{"title":"Insight into Ca2+- inositol 1,4,5-trisphosphate receptor 2 (IP3R2)-mediated unfolded protein response and apoptosis in scallop Patinopecten yessoensis under high temperature stress","authors":"Wenfei Gu , Xiaoxue Ma , Chuanyan Yang , Dongli Jiang , Hongmei Fan , Lingling Wang , Linsheng Song","doi":"10.1016/j.cbpb.2025.111092","DOIUrl":"10.1016/j.cbpb.2025.111092","url":null,"abstract":"<div><div>Inositol 1,4,5-trisphosphate receptor 2 (IP<sub>3</sub>R2) is an essential Ca<sup>2+</sup> release channel protein located in the endoplasmic reticulum (ER), and plays a significant role in responding to various environmental stimuli. In the present study, the function of IP<sub>3</sub>R2 from Yesso scallop <em>Patinopecten yessoensis</em> (<em>Py</em>IP<sub>3</sub>R2) in regulating the Ca<sup>2+</sup>-mediated unfolded protein response (UPR) and apoptosis after high temperature (25 °C) treatment was investigated. Three MIR domains, one RYDR_ITPR domain, one RIH_assoc domain and one Ion_trans domain were identified in <em>Py</em>IP<sub>3</sub>R2. Both D-myo-inositol-1,4,5-triphosphate (IP3, an activator of IP<sub>3</sub>R) and high temperature significantly upregulated the mRNA expression level of <em>PyIP</em><sub><em>3</em></sub><em>R2</em> and genes related to apoptosis and the UPR, and also increased intracellular Ca<sup>2+</sup> content (<em>p</em> < 0.05). Furthermore, the IP<sub>3</sub>R antagonist 2-aminoethyl diphenylborinate (2-APB) had the opposite effect, decreasing intracellular Ca<sup>2+</sup> content and the mRNA expression level of <em>PyIP</em><sub><em>3</em></sub><em>R2</em>, glucose regulated protein 78 (<em>PyGRP78</em>) and <em>PyCaspase-3</em> (<em>p</em> < 0.05). However, the apoptosis rate and Caspase-3 activity remained comparable to those in the injection control group. These findings indicate that <em>Py</em>IP<sub>3</sub>R2 mediates UPR and apoptosis in scallop haemocytes by regulating Ca<sup>2+</sup>content and distribution, and providing insight into the cellular responses of scallops to high temperature.</div></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":"278 ","pages":"Article 111092"},"PeriodicalIF":1.9,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143733326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes in the biochemical and gene expression profiles of Palaemon carinicauda during ovarian development Palaemon carinicauda卵巢发育过程中生化和基因表达谱的变化。

IF 1.9 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-03-18 DOI: 10.1016/j.cbpb.2025.111093

Weitao Cheng , Chao Wang , Dexue Liu , Menghao Fan , Yanrong Cao , Wanyu Han , Tinglu Hu , Guangwei Hu , Huan Gao

Palaemon carinicauda is an economically important shrimp species in China. In this study, muscle, hepatopancreas and ovaries were collected to measure their biochemical composition during ovarian development. In addition, dynamic changes in the transcriptome of P. carinicauda ovaries were investigated. The results showed steady increases in glycogen, triglyceride (TG) and protein levels in the ovaries during maturation. The glycogen level increased consistently in the hepatopancreas, while in the muscle, glycogen showed a downward trend after reaching its highest value at Stage III. Similarly, TG concentration in the hepatopancreas decreased before subsequently increasing, while in the case of muscle, the TG level increased from Stage I to Stage IV prior to a significant decrease in Stage V. In hepatopancreas and muscle, the highest level of protein was observed at Stage III and Stage II respectively and at subsequent stages, the levels decreased, with the lowest value reached in Stage V. Results of RNA-seq revealed dynamic changes in the ovarian transcriptome during development, with extensive changes taking place from Stage I to Stage II and Stage IV to Stage V. KEGG pathway enrichment analysis of upregulated differentially expressed genes (DEGs) revealed that metabolic pathway, lysosome, protein processing in the endoplasmic reticulum, mitogen-activated protein kinase (MAPK) signaling pathway, extracellular matrix (ECM)-receptor interaction, purine metabolism and phagosome were the main enriched pathways. The above results provide a framework for understanding the biochemical and transcriptome dynamics during P. carinicauda ovarian development.

{"title":"Changes in the biochemical and gene expression profiles of Palaemon carinicauda during ovarian development","authors":"Weitao Cheng , Chao Wang , Dexue Liu , Menghao Fan , Yanrong Cao , Wanyu Han , Tinglu Hu , Guangwei Hu , Huan Gao","doi":"10.1016/j.cbpb.2025.111093","DOIUrl":"10.1016/j.cbpb.2025.111093","url":null,"abstract":"<div><div><em>Palaemon carinicauda</em> is an economically important shrimp species in China. In this study, muscle, hepatopancreas and ovaries were collected to measure their biochemical composition during ovarian development. In addition, dynamic changes in the transcriptome of <em>P. carinicauda</em> ovaries were investigated. The results showed steady increases in glycogen, triglyceride (TG) and protein levels in the ovaries during maturation. The glycogen level increased consistently in the hepatopancreas, while in the muscle, glycogen showed a downward trend after reaching its highest value at Stage III. Similarly, TG concentration in the hepatopancreas decreased before subsequently increasing, while in the case of muscle, the TG level increased from Stage I to Stage IV prior to a significant decrease in Stage V. In hepatopancreas and muscle, the highest level of protein was observed at Stage III and Stage II respectively and at subsequent stages, the levels decreased, with the lowest value reached in Stage V. Results of RNA-seq revealed dynamic changes in the ovarian transcriptome during development, with extensive changes taking place from Stage I to Stage II and Stage IV to Stage V. KEGG pathway enrichment analysis of upregulated differentially expressed genes (DEGs) revealed that metabolic pathway, lysosome, protein processing in the endoplasmic reticulum, mitogen-activated protein kinase (MAPK) signaling pathway, extracellular matrix (ECM)-receptor interaction, purine metabolism and phagosome were the main enriched pathways. The above results provide a framework for understanding the biochemical and transcriptome dynamics during <em>P. carinicauda</em> ovarian development.</div></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":"278 ","pages":"Article 111093"},"PeriodicalIF":1.9,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143671885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editorial: Methods in Comparative Biochemistry and Physiology – Challenges and Opportunities

IF 1.9 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-03-15 DOI: 10.1016/j.cbpb.2025.111094

B.G. Borowiec , W. Joyce , C.N. Glover

引用次数: 0

Effects of air exposure time on tissue morphology, energy metabolism, and non-specific immunity of the juvenile Chinese mitten crab, Eriocheir sinensis 空气暴露时间对中华绒螯蟹幼体组织形态、能量代谢和非特异性免疫的影响

IF 1.9 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-03-11 DOI: 10.1016/j.cbpb.2025.111090

Aoxi Zhao , Qinyuan Zhang , Yunfei Sun , Yongxu Cheng

This study investigated the effect of aerial exposure duration (0, 24, 48, and 72 h) on the juvenile Chinese mitten crab, Eriocheir sinensis. The effects on histological structure, energy metabolism, non-specific immunity, and expression of cytochrome P450 (CYP2) and protein kinase B (AKT) genes in the anterior gill and hepatopancreas were analyzed. Longer durations of air exposure led to loss of anterior gill filament shape, and enlargement and deformation of the hepatopancreatic tubules with vacuolization. Pyruvate kinase activity in the hemolymph peaked, and was the lowest in the anterior gill, at 72 h. Lactate dehydrogenase activity and lactic acid content in the hemolymph and anterior gills peaked at 72 h of air exposure. Triglyceride content in hemolymph and anterior gill first increased, then decreased. Superoxide dismutase activity and total antioxidant capacity increased in the hemolymph but initially increased and then decreased in the hepatopancreas, peaking at 24 h. Hemolymph and hepatopancreas acid phosphatase activity peaked at 48 h and in these same tissues lysozyme activity declined with prolonged air exposure. The relative expression of CYP2 and AKT increased during exposure to air in both gill and hepatopancreas. Air exposure exceeding 48 h may affect tissue morphology, energy metabolism, oxidative stress, and immunity of juvenile E. sinensis. Juvenile crabs alter their energy utilization, antioxidant, and metabolic capacities to acclimate to air exposure stress. Therefore, the air exposure time for juvenile E. sinensis should be limited to 48 h during transportation.

{"title":"Effects of air exposure time on tissue morphology, energy metabolism, and non-specific immunity of the juvenile Chinese mitten crab, Eriocheir sinensis","authors":"Aoxi Zhao , Qinyuan Zhang , Yunfei Sun , Yongxu Cheng","doi":"10.1016/j.cbpb.2025.111090","DOIUrl":"10.1016/j.cbpb.2025.111090","url":null,"abstract":"<div><div>This study investigated the effect of aerial exposure duration (0, 24, 48, and 72 h) on the juvenile Chinese mitten crab, <em>Eriocheir sinensis</em>. The effects on histological structure, energy metabolism, non-specific immunity, and expression of cytochrome P450 (<em>CYP2</em>) and protein kinase B (<em>AKT</em>) genes in the anterior gill and hepatopancreas were analyzed. Longer durations of air exposure led to loss of anterior gill filament shape, and enlargement and deformation of the hepatopancreatic tubules with vacuolization. Pyruvate kinase activity in the hemolymph peaked, and was the lowest in the anterior gill, at 72 h. Lactate dehydrogenase activity and lactic acid content in the hemolymph and anterior gills peaked at 72 h of air exposure. Triglyceride content in hemolymph and anterior gill first increased, then decreased. Superoxide dismutase activity and total antioxidant capacity increased in the hemolymph but initially increased and then decreased in the hepatopancreas, peaking at 24 h. Hemolymph and hepatopancreas acid phosphatase activity peaked at 48 h and in these same tissues lysozyme activity declined with prolonged air exposure. The relative expression of <em>CYP2</em> and <em>AKT</em> increased during exposure to air in both gill and hepatopancreas. Air exposure exceeding 48 h may affect tissue morphology, energy metabolism, oxidative stress, and immunity of juvenile <em>E. sinensis</em>. Juvenile crabs alter their energy utilization, antioxidant, and metabolic capacities to acclimate to air exposure stress. Therefore, the air exposure time for juvenile <em>E. sinensis</em> should be limited to 48 h during transportation.</div></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":"278 ","pages":"Article 111090"},"PeriodicalIF":1.9,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143626952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamics of tissue minerals reveal the priority deposition of ovarian minerals during ovarian maturation of Chinese mitten crab Eriocheir sinensis 组织矿物质的动态变化揭示了中华绒螯蟹卵巢成熟过程中卵巢矿物质的优先沉积。

IF 1.9 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-03-11 DOI: 10.1016/j.cbpb.2025.111091

Min Zhang , Qing Guo , Wangling Zhu , Erchao Li , Xugan Wu

Minerals are essential for crustacean growth, development, and reproduction. This study investigated fluctuations in concentrations of twelve minerals in the hepatopancreas, ovary, and muscle of adult female Chinese mitten crabs (Eriocheir sinensis) throughout ovarian maturation (stages II-V), and explored potential mineral transfers between tissues and associated mineral-related gene expression patterns. Results showed a significant increase in five macro-minerals (Na, K, Ca, P, Mg) and most trace minerals (except Fe, Co, Mo) in the ovary (P < 0.05). In contrast, most minerals in the hepatopancreas, including Ca, P, Zn, Mn, Se, Co, and Mo, decreased significantly, while Fe and Cu concentrations increased markedly (P < 0.05). Muscular minerals exhibited varied trends, with Na, K, and Mg increasing significantly (P < 0.05). Ovarian mineral concentrations were positively correlated with the gonadosomatic index (GSI) (P < 0.001), while most hepatopancreatic minerals exhibited a negative correlation with GSI (P < 0.001). Significant correlations were also observed between different minerals in the hepatopancreas, ovary, or muscle (P < 0.05). Additionally, mineral-related genes mt-1β (metallothionein-1β-like) and fer (ferritin-like) were upregulated in both the ovaries and hepatopancreas, showing negative correlations with Ca, P, Mg, Zn, and Mn in the hepatopancreas (P < 0.05) and positive correlations with K, Ca, P, Mg, Cu, Zn, Mn, and Se in the ovary (P < 0.01). In conclusion, the major findings suggest that most minerals are dynamically accumulated in ovary and muscle during the ovarian maturation cycle of E. sinensis, with a potential mineral accumulation and transfer mechanism from the hepatopancreas to the ovary.

{"title":"Dynamics of tissue minerals reveal the priority deposition of ovarian minerals during ovarian maturation of Chinese mitten crab Eriocheir sinensis","authors":"Min Zhang , Qing Guo , Wangling Zhu , Erchao Li , Xugan Wu","doi":"10.1016/j.cbpb.2025.111091","DOIUrl":"10.1016/j.cbpb.2025.111091","url":null,"abstract":"<div><div>Minerals are essential for crustacean growth, development, and reproduction. This study investigated fluctuations in concentrations of twelve minerals in the hepatopancreas, ovary, and muscle of adult female Chinese mitten crabs (<em>Eriocheir sinensis</em>) throughout ovarian maturation (stages II-V), and explored potential mineral transfers between tissues and associated mineral-related gene expression patterns. Results showed a significant increase in five macro-minerals (Na, K, Ca, P, Mg) and most trace minerals (except Fe, Co, Mo) in the ovary (<em>P</em> < 0.05). In contrast, most minerals in the hepatopancreas, including Ca, P, Zn, Mn, Se, Co, and Mo, decreased significantly, while Fe and Cu concentrations increased markedly (<em>P</em> < 0.05). Muscular minerals exhibited varied trends, with Na, K, and Mg increasing significantly (<em>P</em> < 0.05). Ovarian mineral concentrations were positively correlated with the gonadosomatic index (GSI) (<em>P</em> < 0.001), while most hepatopancreatic minerals exhibited a negative correlation with GSI (<em>P</em> < 0.001). Significant correlations were also observed between different minerals in the hepatopancreas, ovary, or muscle (<em>P</em> < 0.05). Additionally, mineral-related genes <em>mt-1β</em> (metallothionein-1β-like) and <em>fer</em> (ferritin-like) were upregulated in both the ovaries and hepatopancreas, showing negative correlations with Ca, P, Mg, Zn, and Mn in the hepatopancreas (<em>P</em> < 0.05) and positive correlations with K, Ca, P, Mg, Cu, Zn, Mn, and Se in the ovary (<em>P</em> < 0.01). In conclusion, the major findings suggest that most minerals are dynamically accumulated in ovary and muscle during the ovarian maturation cycle of <em>E. sinensis,</em> with a potential mineral accumulation and transfer mechanism from the hepatopancreas to the ovary.</div></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":"278 ","pages":"Article 111091"},"PeriodicalIF":1.9,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143626949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Purslane leaf powder dietary supplementation rescues cadmium-induced disruption of behavior, antioxidant status, and expression of tight junction genes, in the brain of Nile tilapia (Oreochromis niloticus)

IF 1.9 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-02-23 DOI: 10.1016/j.cbpb.2025.111086

Nawal Alsubaie , Yasmina M. Abd-Elhakim , Amany Abdel-Rahman Mohamed , Rowida E. Ibrahim , Mohamed M.M. Metwally , Tarek Khamis , Alaa S. Alhegaili , Abd Elhakeem El-Murr , Badriyah S. Alotaibi , Ahmed Abdulwahab Bawahab

This study examined the effects of supplementing the Nile tilapia (Oreochromis niloticus) diet with purslane (Portulaca oleracea L.) leaf powder (PLNP, 10 g/kg) on neurobehavioral performance, brain oxidative status, tight junction mRNA expression, and brain histology in fish exposed to waterborne cadmium (Cd, 50 μg /L) for 60 days. Adding PLNP to the diet ameliorated the Cd-induced decline in ingestive behavior and swimming behavior, and reversed the Cd-induced increase in aggressive behavior. The significant decrease in the non-enzymatic (reduced glutathione) and enzymatic (catalase and superoxide dismutase) brain antioxidants detected in Cd-exposed fish was eliminated by dietary PLNP. PLNP supplementation also led to a decrease in brain malondialdehyde content, which was elevated by Cd exposure. In addition, dietary PLNP increased brain acetylcholinesterase content, upregulated mRNA expression of tight junction (zo-2, claudin-4, and zo-1) and oxidative stress genes (sod-2, gpx, and nrf-2), and downregulated apoptotic genes (p53, caspase-9, caspase-8, and caspase-3) in the brain, relative to the alterations in these parameters caused by Cd exposure. Furthermore, the Cd-induced histological changes in the Nile tilapia brain were ameliorated by PLNP dietary supplementation. In light of these findings, PLNP may be a useful dietary supplement for reducing the harmful effects of Cd on the brain and behavior of Nile tilapia.

{"title":"Purslane leaf powder dietary supplementation rescues cadmium-induced disruption of behavior, antioxidant status, and expression of tight junction genes, in the brain of Nile tilapia (Oreochromis niloticus)","authors":"Nawal Alsubaie , Yasmina M. Abd-Elhakim , Amany Abdel-Rahman Mohamed , Rowida E. Ibrahim , Mohamed M.M. Metwally , Tarek Khamis , Alaa S. Alhegaili , Abd Elhakeem El-Murr , Badriyah S. Alotaibi , Ahmed Abdulwahab Bawahab","doi":"10.1016/j.cbpb.2025.111086","DOIUrl":"10.1016/j.cbpb.2025.111086","url":null,"abstract":"<div><div>This study examined the effects of supplementing the Nile tilapia (<em>Oreochromis niloticus)</em> diet with purslane (<em>Portulaca oleracea</em> L.) leaf powder (PLNP, 10 g/kg) on neurobehavioral performance, brain oxidative status, tight junction mRNA expression, and brain histology in fish exposed to waterborne cadmium (Cd, 50 μg /L) for 60 days. Adding PLNP to the diet ameliorated the Cd-induced decline in ingestive behavior and swimming behavior, and reversed the Cd-induced increase in aggressive behavior. The significant decrease in the non-enzymatic (reduced glutathione) and enzymatic (catalase and superoxide dismutase) brain antioxidants detected in Cd-exposed fish was eliminated by dietary PLNP. PLNP supplementation also led to a decrease in brain malondialdehyde content, which was elevated by Cd exposure. In addition, dietary PLNP increased brain acetylcholinesterase content, upregulated mRNA expression of tight junction (<em>zo-2</em>, <em>claudin-4</em>, and <em>zo-1</em>) and oxidative stress genes (<em>sod-2</em>, <em>gpx</em>, and <em>nrf-2</em>), and downregulated apoptotic genes (<em>p53</em>, <em>caspase-9</em>, <em>caspase-8</em>, and <em>caspase-3</em>) in the brain, relative to the alterations in these parameters caused by Cd exposure. Furthermore, the Cd-induced histological changes in the Nile tilapia brain were ameliorated by PLNP dietary supplementation. In light of these findings, PLNP may be a useful dietary supplement for reducing the harmful effects of Cd on the brain and behavior of Nile tilapia.</div></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":"278 ","pages":"Article 111086"},"PeriodicalIF":1.9,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143506201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Docosapentaenoic (22:5 n-6) and docosahexaenoic (22:6 n-3) acids exhibit highly lipogenic properties in rainbow trout preadipocytes

IF 1.9 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-02-22 DOI: 10.1016/j.cbpb.2025.111087

Gilles Tinant, Ineke Neefs, Alice De Groote, Melissa M. Page, Jean-François Rees, Yvan Larondelle, Cathy Debier

Dietary polyunsaturated fatty acids are essential for fish health. Adipose tissue is the major tissue for fatty acid storage in rainbow trout (Oncorhynchus mykiss), and its development and function can be impacted by the fatty acids themselves. In the present study, the effects of seven fatty acids, oleic (OA, 18:1 n-9), α-linolenic (ALA, 18:3 n-3), eicosapentaenoic (EPA, 20:5 n-3), docosahexaenoic (DHA, 22:6 n-3), linoleic (LA, 18:2 n-6), arachidonic (AA, 20:4 n-6), and docosapentaenoic (DPA, 22:5 n-6) acids, on adipogenesis were investigated in primary cultures of rainbow trout preadipocytes. In terms of lipid accumulation, DPA and DHA appeared to be the most lipogenic fatty acids, while all treatments modified the fatty acid composition of the cellular phospholipids and neutral lipids. The fatty acid of interest added to the culture medium was the most abundant in preadipocytes, while the first bioconversion products were detected in lower amounts. In terms of transcriptional effects, DPA increased the expression of the early transcription factor CCAAT/enhancer binding protein δ, while DHA upregulated the expression of genes involved in neutral lipid synthesis, notably lipoprotein lipase, fatty acid transport protein 1 and glycerol-3-phosphate dehydrogenase. Both fatty acids decreased the expression of fatty acid synthase. These results highlight that DPA and DHA exert a significant effect on lipid deposition in rainbow trout preadipocytes, potentially through different pathways, and confirm that fatty acids have major impacts on preadipocyte lipid metabolism and adipogenesis.

{"title":"Docosapentaenoic (22:5 n-6) and docosahexaenoic (22:6 n-3) acids exhibit highly lipogenic properties in rainbow trout preadipocytes","authors":"Gilles Tinant, Ineke Neefs, Alice De Groote, Melissa M. Page, Jean-François Rees, Yvan Larondelle, Cathy Debier","doi":"10.1016/j.cbpb.2025.111087","DOIUrl":"10.1016/j.cbpb.2025.111087","url":null,"abstract":"<div><div>Dietary polyunsaturated fatty acids are essential for fish health. Adipose tissue is the major tissue for fatty acid storage in rainbow trout (<em>Oncorhynchus mykiss</em>), and its development and function can be impacted by the fatty acids themselves. In the present study, the effects of seven fatty acids, oleic (OA, 18:1 n-9), α-linolenic (ALA, 18:3 n-3), eicosapentaenoic (EPA, 20:5 n-3), docosahexaenoic (DHA, 22:6 n-3), linoleic (LA, 18:2 n-6), arachidonic (AA, 20:4 n-6), and docosapentaenoic (DPA, 22:5 n-6) acids, on adipogenesis were investigated in primary cultures of rainbow trout preadipocytes. In terms of lipid accumulation, DPA and DHA appeared to be the most lipogenic fatty acids, while all treatments modified the fatty acid composition of the cellular phospholipids and neutral lipids. The fatty acid of interest added to the culture medium was the most abundant in preadipocytes, while the first bioconversion products were detected in lower amounts. In terms of transcriptional effects, DPA increased the expression of the early transcription factor CCAAT/enhancer binding protein δ, while DHA upregulated the expression of genes involved in neutral lipid synthesis, notably lipoprotein lipase, fatty acid transport protein 1 and glycerol-3-phosphate dehydrogenase. Both fatty acids decreased the expression of fatty acid synthase. These results highlight that DPA and DHA exert a significant effect on lipid deposition in rainbow trout preadipocytes, potentially through different pathways, and confirm that fatty acids have major impacts on preadipocyte lipid metabolism and adipogenesis.</div></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":"278 ","pages":"Article 111087"},"PeriodicalIF":1.9,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0