HOS15 impacts DIL9 protein stability during drought stress in Arabidopsis

IF 8.1 1区生物学 Q1 PLANT SCIENCES New Phytologist Pub Date : 2025-01-31 DOI:10.1111/nph.20398

Shah Zareen, Akhtar Ali, Junghoon Park, Sang-Mo Kang, In-Jung Lee, Jose M. Pardo, Dae-Jin Yun, Zheng-Yi Xu

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Abstract

HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENE 15 (HOS15) acts as a substrate receptor of E3 ligase complex, which plays a negative role in drought stress tolerance. However, whether and how HOS15 participates in controlling important transcriptional regulators remains largely unknown.
Here, we report that HOS15 physically interacts with and tightly regulates DROUGHT-INDUCED LIKE 19 (DIL9) protein stability. Moreover, application of exogenous abscisic acid (ABA) stabilizes the interaction between DIL9 and HOS15, leading to ABA-induced proteasomal degradation of DIL9 by HOS15. Genetic analysis revealed that DIL9 functions downstream to HOS15 and that the drought tolerance of hos15-2 plants was impaired in dil9/hos15 double mutants.
Notably, DIL9 is directly associated with the promoter regions of ABF transcription factors and facilitates their expression, which is pivotal in enhancing ABA-dependent drought tolerance.
Collectively, these findings demonstrate that HOS15 consistently degrades DIL9 under normal condition, while stress (drought/ABA) promotes the DIL9 activity for binding to the promoter regions of ABFs and positively regulates their expression in response to dehydration.

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干旱胁迫下HOS15影响拟南芥DIL9蛋白稳定性

高表达的渗透反应基因15 （OSMOTICALLY RESPONSIVE GENE 15, HOS15）是E3连接酶复合物的底物受体，在干旱胁迫耐受中起负作用。然而，HOS15是否以及如何参与控制重要的转录调控因子在很大程度上仍然未知。在这里，我们报道了HOS15与干旱诱导的LIKE 19 （DIL9）蛋白的物理相互作用并严格调节其稳定性。此外，外源脱落酸（ABA）的应用稳定了DIL9和HOS15之间的相互作用，导致ABA诱导的蛋白酶体降解DIL9被HOS15降解。遗传分析表明DIL9在HOS15的下游发挥作用，DIL9 / HOS15双突变体对HOS15 -2植株的耐旱性降低。值得注意的是，DIL9与ABF转录因子的启动子区直接相关并促进其表达，这在增强aba依赖性抗旱性中起着关键作用。综上所述，这些研究结果表明，在正常条件下，HOS15持续降解DIL9，而胁迫（干旱/ABA）促进DIL9与abf启动子区域结合的活性，并在脱水条件下积极调节其表达。

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.