Wenqing Wang, Elizabeth R Wong, Michael L Cato, Patrick C Daly, Chandler G Doody, Townshend Gillespie, Caroline N Ksor, Arooj Zainab, Zunaira Zainab, Matt Crook
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引用次数: 0
Abstract
Quick Oil Red O staining is a well established method to assay total lipid levels in Caenorhabditis elegans , but the software to clean up and analyse the images is either laborious, expensive or both. We have developed a process that uses an existing protocol to stain the animals, followed by Magic Select in Paint3D to remove background and then a custom script in Biopython to quantify average pixel intensity per animal. The software is free, accessible and relatively easy to use for undergraduate researchers.
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