A novel range-divided data dependent acquisition strategy for screening of diterpenoid alkaloids in Aconitum pendulum roots

IF 2.8 3区医学 Q2 BIOCHEMICAL RESEARCH METHODS Journal of Chromatography B Pub Date : 2025-01-27 DOI:10.1016/j.jchromb.2025.124486

Mei Deng , Bishi Ren , Jiayi Yi , Hui Ding , Hua Wang

引用次数: 0

Abstract

A novel range-divided data dependent acquisition (DDA) strategy was proposed for the screening of diterpenoid alkaloids in Aconitum pendulum roots. In range-divided DDA, the low-range was set between m/z 340–500 and the high-range was set between m/z 500–700 according to the molecular weight range of the diterpenoid alkaloids. The combined identification approach including MS¹ molecular weight, MS² spectrum interpretation, literature comparison, and standard verification was applied to the results. The range-divided DDA identified 15 more diterpenoid alkaloids than the full-range DDA under the same LC conditions. A total of 47 diterpenoid alkaloids were identified. Among them, brachyaconitines A-D were screened for the first time in Aconitum pendulum. This screening strategy can serve as a powerful tool for the discovery of novel metabolites in the field of plant metabolomics.

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一种新颖的范围划分数据依赖采集策略，用于筛选摆尾草根中的二萜生物碱。

提出了一种新的距离分割数据依赖采集（DDA）方法，用于乌头摆根中二萜类生物碱的筛选。在区间划分DDA中，根据二萜类生物碱的分子量范围，下限设定在m/z 340 ~ 500之间，上限设定在m/z 500 ~ 700之间。采用MS1分子量、MS2光谱解释、文献比对、标准品验证等联合鉴定方法。在相同的LC条件下，范围划分DDA比全范围DDA多鉴定出15种二萜类生物碱。共鉴定出47种二萜类生物碱。其中，短乌头碱A-D为首次从乌头中分离得到。该筛选策略可作为植物代谢组学领域发现新型代谢物的有力工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Chromatography B 医学-分析化学

CiteScore

5.60

自引率

3.30%

发文量

306

审稿时长

44 days

期刊介绍： The Journal of Chromatography B publishes papers on developments in separation science relevant to biology and biomedical research including both fundamental advances and applications. Analytical techniques which may be considered include the various facets of chromatography, electrophoresis and related methods, affinity and immunoaffinity-based methodologies, hyphenated and other multi-dimensional techniques, and microanalytical approaches. The journal also considers articles reporting developments in sample preparation, detection techniques including mass spectrometry, and data handling and analysis. Developments related to preparative separations for the isolation and purification of components of biological systems may be published, including chromatographic and electrophoretic methods, affinity separations, field flow fractionation and other preparative approaches. Applications to the analysis of biological systems and samples will be considered when the analytical science contains a significant element of novelty, e.g. a new approach to the separation of a compound, novel combination of analytical techniques, or significantly improved analytical performance.