Mitigation of salinity stress in salt-sensitive rice seedlings via phytohormone synthesis, antioxidant defence enhancement, and ion balance regulation induced by 5-aminolevulinic acid-producing purple non-sulfur bacteria.

Abstract

Salt stress, intensified by climate change, is a significant threat to rice production, a vital staple for over half the world's population. This makes addressing salt stress in rice cultivation a pressing issue. This study investigates the role of PNSB as a biostimulant in enhancing salinity tolerance of salt-sensitive rice seedlings, addressing existing gaps in knowledge on physiological and biochemical impacts under saline stress. We inoculated salt-sensitive rice seedlings with PNSB under 80 mmol NaCl stress in a controlled environment. After a 5-day treatment, we conducted biochemical and physiological analyses. Salinity stress induced oxidative stress in salt-sensitive rice seedlings. However, application of 5-ALA-producing PNSB mitigated stress, elevated 5-ALA in shoots by 23%, roots by 190.5%, and chlorophyll content by 105.0%. PNSB treatment also reduced superoxide radicals (O₂ ^•-) and H₂O₂ by 26.7% and 38.7%, respectively, related to increased activity of the antioxidant enzymes, SOD (142.9%) and APX (41.8%). This led to lower electrolyte leakage (25.2%) and MDA (17.4%), indicating reduced ROS. Additionally, proline and soluble sugar content decreased by 29.2% and 72.5%, respectively. PNSB treatment also reduced sodium to potassium ion content in both shoots (31.2%) and roots (27.4%) of salt-stressed rice seedlings. These findings suggest that PNSB may facilitate nutrient solubilization and ion balance, thereby mitigating the adverse effects of salinity, with potential implications for sustainable agricultural practices to improve crop yield in saline environments. Future research should focus on elucidating the specific biochemical pathways involved in PNSB-mediated stress tolerance and exploring their application across diverse crop species and varying stress conditions.