Rapid and effective removal of 2,4-dichlorophenol by peroxidase from potato.

Abstract

Phenolic compounds discharged in environmental water are required to remove as soon as possible by using the effective methods such as peroxidase catalyzed oxidation to avoid harm to human health. The crude peroxidase is sectionally salted out of potato starch processing wastewater by 40-70% saturated ammonium sulphate, and used to catalyze oxidation of 2,4-dichlorophenol by hydrogen peroxide. Results show that the potato peroxidase has a fine catalytic effect on the oxidation of hydrogen peroxide at 20-50 ℃ and pH 3-10. In just 10 min, 98% of phenol compound can be effectively removed from 1 mL of reaction system containing 1.0 mmol/L 2,4-dichlorophenol, 1.2 mmol/L H₂O₂ and 50 U/mL peroxidase at pH 6. Obviously, this peroxidase catalyzed oxidation is a green, rapid and effective way, having great potential for removing 2-4-dichlorophenol from polluted water bodies.