Injectable Self-Assembling Procyanidin Nanospheres for Effective Osteoarthritis Treatment.

IF 6.5 2区医学 Q1 NANOSCIENCE & NANOTECHNOLOGY International Journal of Nanomedicine Pub Date : 2025-01-29 eCollection Date: 2025-01-01 DOI:10.2147/IJN.S496827

Guangjie Li, Fei He, Jianbao Feng, Ge Xu, Chengye Wu, Yufei Qiao, Yang Liu, Hanlin Chen, Pengcheng Du, Jizeng Wang

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Abstract

Background: Osteoarthritis (OA), a prevalent joint disease, causes immense suffering to thousands of patients, impairing their mobility and diminishing their quality of life. Current treatment methods primarily rely on analgesics or anti-inflammatory drugs to alleviate symptoms but fail to achieve the desired therapeutic outcome.

Methods: To better realize therapeutic effects of OA, procyanidins (PAs), as a type of plant flavonoids with strong antioxidant and anti-inflammatory activities, were designed to self-assembly with well-dispersible Pluronic F127 (PF127) through the hydrogen-bond interaction to present an injectable, biocompatibility PA nanospheres.

Results: These nanospheres significantly increased the cell viability in mouse L929 fibroblasts and ADTC5 chondrocytes compared with unassembled PAs. In addition, the self-assembling PAs/PF127 nanospheres enhanced the protein expression of collagen (COL1A1 and COL3A1) in fibroblasts, and the expression of glycosaminoglycan and COL2A1 was also higher than unassembled PAs in chondrocytes, this heralded the potential to achieve OA repair strategies at the cellular level. In an enzymolysis model of rat OA, PAs/PF127 nanospheres significantly reduce joint space swelling in the early stages of cartilage destruction and accelerate the formation of subchondral bone and cartilaginous surface.

Implication: This study offers valuable insights into the preparation of novel PA nanospheres for effective repair of OA.

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可注射自组装原花青素纳米球有效治疗骨关节炎。

背景：骨关节炎（OA）是一种常见的关节疾病，给成千上万的患者带来了巨大的痛苦，损害了他们的活动能力，降低了他们的生活质量。目前的治疗方法主要依靠镇痛药或抗炎药来缓解症状，但未能达到预期的治疗效果。方法：为了更好地发挥OA的治疗作用，将原花青素（procyanidins, PAs）作为一类具有较强抗氧化和抗炎活性的植物黄酮类化合物，通过氢键相互作用与分散性良好的Pluronic F127 （PF127）进行自组装，制备可注射的生物相容性良好的PA纳米微球。结果：与未组装的PAs相比，这些纳米微球显著提高了小鼠L929成纤维细胞和ADTC5软骨细胞的细胞活力。此外，自组装PAs/PF127纳米球增强了成纤维细胞中胶原蛋白（COL1A1和COL3A1）的表达，糖胺聚糖和COL2A1的表达也高于软骨细胞中未组装PAs的表达，这预示着在细胞水平上实现OA修复策略的潜力。在大鼠OA酶解模型中，PAs/PF127纳米微球显著减少软骨破坏早期关节间隙肿胀，加速软骨下骨和软骨表面的形成。意义：本研究为新型PA纳米球的制备提供了有价值的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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文献相关原料

公司名称

产品信息

索莱宝

4% papain

索莱宝

6 M guanidine-HCl

索莱宝

4% formaldehyde

索莱宝

alcian blue

索莱宝

penicillin/streptomycin solution

来源期刊

International Journal of Nanomedicine NANOSCIENCE & NANOTECHNOLOGY-PHARMACOLOGY & PHARMACY

CiteScore

14.40

自引率

3.80%

发文量

511

审稿时长

1.4 months

期刊介绍： The International Journal of Nanomedicine is a globally recognized journal that focuses on the applications of nanotechnology in the biomedical field. It is a peer-reviewed and open-access publication that covers diverse aspects of this rapidly evolving research area. With its strong emphasis on the clinical potential of nanoparticles in disease diagnostics, prevention, and treatment, the journal aims to showcase cutting-edge research and development in the field. Starting from now, the International Journal of Nanomedicine will not accept meta-analyses for publication.