Tersicoccus mangrovi sp. nov., a novel actinobacterium from mangrove sediment in Semarang city, Indonesia.

Abstract

A Gram-stain-positive, aerobic and non-motile actinobacterium, designated strain MR15.9^T, was isolated from sediment collected from a mangrove ecosystem in Semarang city, Indonesia. Strain MR15.9^T grew at 4-37 °C (optimum 30 °C), pH 6.0-11.0 (optimum 7.0-8.0), with 0-10% (w/v) NaCl (optimum 0-3%). The genome of strain MR15.9^T was 3.67 Mbp with 71.7 mol% G+C content. Phylogenetic analysis based on 16S rRNA gene sequence and genome sequence directed that strain MR15.9^T formed a well-supported clade with Tersicoccus solisilvae CGMCC 1.15480^T and Tersicoccus phoenicis 1P05MA^T and shared the highest similarity to T. solisilvae CGMCC 1.15480^T (98.9% sequence similarity) and T. phoenicis 1P05MA^T (98.6% sequence similarity). However, the comparative genome analysis between strain MR15.9^T and T. solisilvae CGMCC 1.15480^T gave average nt identity value of 85.2% and digital DNA-DNA hybridization value of 29.0%, which were below the statistical threshold for the delineation of the species. Chemotaxonomic data showed that the major fatty acids were anteiso-C_15:0, anteiso-C_17:0 and iso-C_16:0. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two unidentified glycolipids and two unidentified phospholipids. MK-8(H₂) and MK-9(H₂) were noted as the predominant respiratory quinones in this strain. The results of polyphasic characterization indicated that strain MR15.9^T represents a novel species of the genus Tersicoccus. The name Tersicoccus mangrovi sp. nov. is proposed, with the type strain MR15.9^T (=MCCC 1K08875^T=KCTC 59105^T).