A strategy integrated DNA barcoding with metabolomics for screening distinguishable combinatorial chemical quality marker between Pheretima aspergillum and Pheretima vulgaris Chen
Ye Shang , Suyi Liu , Chunxiao Liang , Tenukeguli Tuliebieke , Shujing Chen , Kunze Du , Xiaoxuan Tian , Jin Li , Jun He , Hua Jin , Yanxu Chang
{"title":"A strategy integrated DNA barcoding with metabolomics for screening distinguishable combinatorial chemical quality marker between Pheretima aspergillum and Pheretima vulgaris Chen","authors":"Ye Shang , Suyi Liu , Chunxiao Liang , Tenukeguli Tuliebieke , Shujing Chen , Kunze Du , Xiaoxuan Tian , Jin Li , Jun He , Hua Jin , Yanxu Chang","doi":"10.1016/j.jpba.2025.116716","DOIUrl":null,"url":null,"abstract":"<div><div><em>Pheretima</em> is an animal-derived traditional Chinese medicines (TCMs). The chemical quality markers of <em>Pheretima</em> used to distinguish different species are still ambiguous. Under this premise, a strategy integrated DNA barcoding with metabolomics is promoted for identifying <em>Pheretima</em> and screening distinguishable combinatorial chemical quality marker (DCQ-marker) between <em>Pheretima aspergillum</em> (<em>P. aspergillum</em>) and <em>Pheretima vulgaris</em> Chen (<em>P. vulgaris</em>). As a result, adenosine, adenine, L-phenylalanine and uridine are successfully selected as DCQ-markers between <em>P. aspergillum</em> and <em>P. vulgaris</em>. This study provides convenient strategy for quickly screening DCQ-marker between <em>P. aspergillum</em> and <em>P. vulgaris</em>. It will be meaningful for further promoting quality control on <em>Pheretima</em> and providing a reference for the quality evaluation of other animal-derived TCMs.</div></div>","PeriodicalId":16685,"journal":{"name":"Journal of pharmaceutical and biomedical analysis","volume":"257 ","pages":"Article 116716"},"PeriodicalIF":3.1000,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of pharmaceutical and biomedical analysis","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0731708525000573","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
Pheretima is an animal-derived traditional Chinese medicines (TCMs). The chemical quality markers of Pheretima used to distinguish different species are still ambiguous. Under this premise, a strategy integrated DNA barcoding with metabolomics is promoted for identifying Pheretima and screening distinguishable combinatorial chemical quality marker (DCQ-marker) between Pheretima aspergillum (P. aspergillum) and Pheretima vulgaris Chen (P. vulgaris). As a result, adenosine, adenine, L-phenylalanine and uridine are successfully selected as DCQ-markers between P. aspergillum and P. vulgaris. This study provides convenient strategy for quickly screening DCQ-marker between P. aspergillum and P. vulgaris. It will be meaningful for further promoting quality control on Pheretima and providing a reference for the quality evaluation of other animal-derived TCMs.
黄花是一种动物源性中药。用于区分不同种属的雨葵化学质量标记尚不明确。在此前提下，提出了一种DNA条形码与代谢组学相结合的方法来鉴定假凤蝶，并筛选假凤蝶（phetima aspergillum， P. aspergillum）和假凤蝶（phetima vulgaris Chen， P. vulgaris）可区分的组合化学质量标记（DCQ-marker）。结果表明，腺苷、腺嘌呤、l -苯丙氨酸和尿嘧啶被成功地选择为曲霉与寻常假单胞菌之间的dcq标记。本研究为快速筛选曲霉与寻常假单胞菌之间的dcq标记提供了方便的策略。这对进一步加强黄花药材的质量控制，并为其他动物源性中药的质量评价提供参考意义。
期刊介绍：
This journal is an international medium directed towards the needs of academic, clinical, government and industrial analysis by publishing original research reports and critical reviews on pharmaceutical and biomedical analysis. It covers the interdisciplinary aspects of analysis in the pharmaceutical, biomedical and clinical sciences, including developments in analytical methodology, instrumentation, computation and interpretation. Submissions on novel applications focusing on drug purity and stability studies, pharmacokinetics, therapeutic monitoring, metabolic profiling; drug-related aspects of analytical biochemistry and forensic toxicology; quality assurance in the pharmaceutical industry are also welcome.
Studies from areas of well established and poorly selective methods, such as UV-VIS spectrophotometry (including derivative and multi-wavelength measurements), basic electroanalytical (potentiometric, polarographic and voltammetric) methods, fluorimetry, flow-injection analysis, etc. are accepted for publication in exceptional cases only, if a unique and substantial advantage over presently known systems is demonstrated. The same applies to the assay of simple drug formulations by any kind of methods and the determination of drugs in biological samples based merely on spiked samples. Drug purity/stability studies should contain information on the structure elucidation of the impurities/degradants.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
