A low-cost open-source 3D-printed mouse cradle suspension system for awake or anaesthetised 1H/31P magnetic resonance spectroscopy

IF 2 Q3 ENGINEERING, ELECTRICAL & ELECTRONIC HardwareX Pub Date : 2024-12-30 DOI:10.1016/j.ohx.2024.e00616
Saba Molhemi, Leif Østergaard, Brian Hansen
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Abstract

Awake mouse MRI and spectroscopy (MRS) are valuable techniques for studying biological questions without the confounding effects of anaesthesia. Currently, no off-the-shelf solution exists for awake mouse MRI/S. To address this, we present a Mouse Cradle Suspension System (MCSS) for awake mouse MRI/S. Our design is freely available and offers a low-cost 3D-printed setup compatible with a Bruker Biospec 94/20 scanner and commercially available 1H/31P surface- and volume-coils, such as coils from Bruker Biospin (T20025V3) and Rapid (O-XL-HL-094). While the focus here is measurements in awake mouse brain, the coils and the presented setup is suitable for both mouse and rat brain, and studies of mouse body organs. Moreover, the design is easily modifiable to suit other applications and hardware configurations. The MCSS reduces gradient-induced coil vibrations and supports cross-coil setups. It features an inner and outer rail system for easy insertion of the coil and customized mouse cradle into the scanner. The cradle is suitable for both anaesthetized and awake mouse scans and existing habituation protocols for awake mouse MRI/S. This MCSS design ensures a smooth workflow for awake mouse MRI/S. The cost is approximately 200€, achieved using 3D-printed and off-the-shelf components.

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清醒状态下的小鼠核磁共振成像(MRI)和光谱学(MRS)是研究生物问题的重要技术,而不会受到麻醉的影响。目前,还没有现成的清醒小鼠 MRI/S 解决方案。为了解决这个问题,我们推出了用于清醒小鼠 MRI/S 的小鼠摇篮悬挂系统(MCSS)。我们的设计是免费提供的,它是一种低成本的 3D 打印装置,可与布鲁克 Biospec 94/20 扫描仪和市售的 1 H/31P 表面和体积线圈(如布鲁克 Biospin (T20025V3) 和 Rapid (O-XL-HL-094) 的线圈)兼容。虽然本文的重点是在清醒的小鼠大脑中进行测量,但线圈和所介绍的装置既适用于小鼠和大鼠大脑,也适用于小鼠身体器官的研究。此外,该设计很容易修改,以适应其他应用和硬件配置。MCSS 可减少梯度引起的线圈振动,并支持交叉线圈设置。它采用内外导轨系统,便于将线圈和定制的小鼠托架插入扫描仪。台座既适用于麻醉小鼠扫描,也适用于清醒小鼠扫描,还适用于清醒小鼠 MRI/S 的现有习惯化协议。这种 MCSS 设计可确保清醒小鼠 MRI/S 的工作流程顺畅。成本约为 200 欧元,采用 3D 打印和现成部件。
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来源期刊
HardwareX
HardwareX Engineering-Industrial and Manufacturing Engineering
CiteScore
4.10
自引率
18.20%
发文量
124
审稿时长
24 weeks
期刊介绍: HardwareX is an open access journal established to promote free and open source designing, building and customizing of scientific infrastructure (hardware). HardwareX aims to recognize researchers for the time and effort in developing scientific infrastructure while providing end-users with sufficient information to replicate and validate the advances presented. HardwareX is open to input from all scientific, technological and medical disciplines. Scientific infrastructure will be interpreted in the broadest sense. Including hardware modifications to existing infrastructure, sensors and tools that perform measurements and other functions outside of the traditional lab setting (such as wearables, air/water quality sensors, and low cost alternatives to existing tools), and the creation of wholly new tools for either standard or novel laboratory tasks. Authors are encouraged to submit hardware developments that address all aspects of science, not only the final measurement, for example, enhancements in sample preparation and handling, user safety, and quality control. The use of distributed digital manufacturing strategies (e.g. 3-D printing) is encouraged. All designs must be submitted under an open hardware license.
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