The applications of bio-orthogonal Raman labels for visualizing lipids in eukaryotic cells

IF 11.8 1区化学 Q1 CHEMISTRY, ANALYTICAL Trends in Analytical Chemistry Pub Date : 2025-02-01 DOI:10.1016/j.trac.2024.118099

Ning Xu , Damei Sun , Hongwei Sun , Qiaoling Ding , Jiangyu Zong , Yuchan Qu , Man Hong , Ke Tong

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Abstract

Lipid metabolism is closely associated with atherosclerosis, obesity, diabetes, and many other metabolic diseases. Visualizing the distribution and kinetics of lipids are critical for understanding the role of lipids. Compared with fluorophores, bio-orthogonal Raman labels, such as deuterium and alkynes, are smaller and have unique Raman vibrations in the silent region of the cell. They've been widely used as tags for the cellular lipid metabolism tracking. For Raman tags of deuterium, the molecular mechanisms involved in atherosclerosis formation, lipid droplet doping, lipid storage capacity, and the physical properties of lipids are reviewed. For Raman tags of alkynes, multiple design strategies, including conjugation with multiple aromatic hydrocarbons and isotope labeling, are summarized for stronger signals and multiple imaging. The novel bio-orthogonal Raman tags of nitriles and astaxanthin is also described. The unique advantages of Raman spectroscopy combined with bio-orthogonal Raman labeling have been demonstrated in lipid imaging, and these applications provide new biological insights into lipid metabolism.

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来源期刊

Trends in Analytical Chemistry 化学-分析化学

CiteScore

20.00

自引率

4.60%

发文量

257

审稿时长

3.4 months

期刊介绍： TrAC publishes succinct and critical overviews of recent advancements in analytical chemistry, designed to assist analytical chemists and other users of analytical techniques. These reviews offer excellent, up-to-date, and timely coverage of various topics within analytical chemistry. Encompassing areas such as analytical instrumentation, biomedical analysis, biomolecular analysis, biosensors, chemical analysis, chemometrics, clinical chemistry, drug discovery, environmental analysis and monitoring, food analysis, forensic science, laboratory automation, materials science, metabolomics, pesticide-residue analysis, pharmaceutical analysis, proteomics, surface science, and water analysis and monitoring, these critical reviews provide comprehensive insights for practitioners in the field.