A simple and expeditious RP-HPLC method for the simultaneous determination of commercially available seven pesticides formulations

Abstract

For the purpose of simultaneously determination, a quick, easy, accurate and precise, high-performance liquid chromatographic analytical technique was validated for tolfenpyrad, pyraclostrobin, trifloxystrobin, azoxystrobin, fipronil, thiamethoxam, thiophanate-methyl, and azoxystrobin in their respective commercially accessible technical product compositions. Active components were separated chromatographically from additives and co-formulations using isocratic elution with 0.1 % orthophosphoric acid and acetonitrile in a 30:70 (v/v) at a flow rate of 1.0 mL per min on an Inertsil ODS-3 column (250 × 4.6 mm; 3.5 µm) with UV detection at 190 nm. With a 20-minute run time, the retention times for thiamethoxam, thiophanate-methyl, azoxystrobin, fipronil, pyraclostrobin, trifloxystrobin, and tolfenpyrad were found to be 2.96, 3.68, 5.98, 8.39, 11.31, 13.74, and 16.38 min, respectively. SANCO guidelines for precision, linearity, robustness, accuracy, LOD, specificity and selectivity were followed to approve the efficiency of this technique. Because all analyses were conducted using a single method and constant chromatographic settings, the suggested method offers several advantages, including rapid, sustainable, and eco-friendly analysis, as well as overall low costs. Developed method can be applied to water, food and different sources samples.