A UDP-glucuronosyltransferase gene UGT379A1 involved in detoxification of lufenuron in Diaphorina citri

IF 4 1区农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pesticide Biochemistry and Physiology Pub Date : 2024-12-24 DOI:10.1016/j.pestbp.2024.106260

Ze-Hao Huang , Qi Pan , Zhen Wu , Yu-Chao Shen , Si-Chen Li , Qi-Qi Yang , Shao-Hui Zhang , Shuang Lei , Yang-Yang Cui , Li-Li Ding , Shi-Jiang Yu , Liu Liu , Lin Cong , Bing-Hai Lou , Xue-Feng Wang , Chun Ran , Ling Liao

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Abstract

The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae), is a devastating bacterial disease of commercial citrus. Presently, uridine diphosphate (UDP)-glycosyltransferases (UGTs), have been linked to the detoxification of pesticides, were known as phase II enzymes in the detoxification process. However, the role of UGTs in detoxification of lufenuron in Diaphorina citri is unknown. In this study, we identified a UGT gene, UGT379A1, which was significantly up-regulated under the exposure of lufenuron. The knockdown of UGT379A1 increased the susceptibility of D. citri to lufenuron. In vitro metabolism and Dixon plot analysis indicated that UGT379A1 could deplete lufenuron by sequestration. These results showed that UGT379A1 was involved in the lufenuron detoxification, which provides a theoretical basis for the prevention and control of D. citri.

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糖醛酸苷基转移酶基因UGT379A1参与柑橘地瓜对氟虫腈的解毒作用

亚洲柑橘木虱（Diaphorina citri Kuwayama）（半翅目：木虱科）是一种破坏性的商品柑橘细菌性病害。目前，尿苷二磷酸(UDP)-糖基转移酶（UGTs）被认为是农药解毒过程中的第二阶段酶，与农药的解毒作用有关。然而，ugt在柑桔地虱对氟虫腈解毒中的作用尚不清楚。在本研究中，我们鉴定了UGT基因UGT379A1，该基因在氟虫腈暴露下显著上调。UGT379A1基因的敲低增加了柑橘对氟虫腈的敏感性。体外代谢和Dixon图分析表明，UGT379A1可以通过固存消耗氟虫腈。这些结果表明，UGT379A1参与了氟虫腈的解毒，为柑桔病的防治提供了理论依据。

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来源期刊

Pesticide Biochemistry and Physiology 生物-昆虫学

CiteScore

7.00

自引率

8.50%

发文量

238

审稿时长

4.2 months

期刊介绍： Pesticide Biochemistry and Physiology publishes original scientific articles pertaining to the mode of action of plant protection agents such as insecticides, fungicides, herbicides, and similar compounds, including nonlethal pest control agents, biosynthesis of pheromones, hormones, and plant resistance agents. Manuscripts may include a biochemical, physiological, or molecular study for an understanding of comparative toxicology or selective toxicity of both target and nontarget organisms. Particular interest will be given to studies on the molecular biology of pest control, toxicology, and pesticide resistance. Research Areas Emphasized Include the Biochemistry and Physiology of: • Comparative toxicity • Mode of action • Pathophysiology • Plant growth regulators • Resistance • Other effects of pesticides on both parasites and hosts.