Enzyme-mediated controlled polymerization and its application in biomolecular analysis

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL Microchemical Journal Pub Date : 2025-01-01 DOI:10.1016/j.microc.2024.112581

Shiyu Liu , Nan Ma , Jinming Kong , Xueji Zhang

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Abstract

As an efficient biological catalyst, enzyme has an important role in various biochemical reactions. Enzymes have stronger advantages of regioselectivity and stereoselectivity in enzymatic polymerization than ordinary catalysts, and enzymatic polymerization also displays characteristics of safety, high efficiency and environmentally friendly, so its application in the fields of biomedicine and nanomaterials is gradually expanding. Recently the combination of enzymes and polymers has been developed as an important tool for the detection of biomolecules. This review summarizes the mechanism and development process of enzyme-mediated polymerization and discusses the application of enzyme-mediated controlled polymerization to biomolecular analysis, as well as the importance of enzyme mimics and immobilized enzymes in enzyme-promoted polymerization. Finally, some new trends and important developments in enzymatic polymerization are summarized as well as major challenges and future prospects of its application to biomolecular analysis. Besides, this review emphasizes the use of enzymatic polymerization for the development of biosensors.

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来源期刊

Microchemical Journal 化学-分析化学

CiteScore

8.70

自引率

8.30%

发文量

1131

审稿时长

1.9 months

期刊介绍： The Microchemical Journal is a peer reviewed journal devoted to all aspects and phases of analytical chemistry and chemical analysis. The Microchemical Journal publishes articles which are at the forefront of modern analytical chemistry and cover innovations in the techniques to the finest possible limits. This includes fundamental aspects, instrumentation, new developments, innovative and novel methods and applications including environmental and clinical field. Traditional classical analytical methods such as spectrophotometry and titrimetry as well as established instrumentation methods such as flame and graphite furnace atomic absorption spectrometry, gas chromatography, and modified glassy or carbon electrode electrochemical methods will be considered, provided they show significant improvements and novelty compared to the established methods.