Enzyme-mediated controlled polymerization and its application in biomolecular analysis

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL Microchemical Journal Pub Date : 2025-01-01 Epub Date: 2024-12-25 DOI:10.1016/j.microc.2024.112581

Shiyu Liu , Nan Ma , Jinming Kong , Xueji Zhang

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Abstract

As an efficient biological catalyst, enzyme has an important role in various biochemical reactions. Enzymes have stronger advantages of regioselectivity and stereoselectivity in enzymatic polymerization than ordinary catalysts, and enzymatic polymerization also displays characteristics of safety, high efficiency and environmentally friendly, so its application in the fields of biomedicine and nanomaterials is gradually expanding. Recently the combination of enzymes and polymers has been developed as an important tool for the detection of biomolecules. This review summarizes the mechanism and development process of enzyme-mediated polymerization and discusses the application of enzyme-mediated controlled polymerization to biomolecular analysis, as well as the importance of enzyme mimics and immobilized enzymes in enzyme-promoted polymerization. Finally, some new trends and important developments in enzymatic polymerization are summarized as well as major challenges and future prospects of its application to biomolecular analysis. Besides, this review emphasizes the use of enzymatic polymerization for the development of biosensors.

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酶介导的控制聚合及其在生物分子分析中的应用

酶作为一种高效的生物催化剂，在各种生物化学反应中起着重要的作用。酶在酶促聚合中具有比普通催化剂更强的区域选择性和立体选择性优势，同时酶促聚合也表现出安全、高效、环保的特点，因此在生物医药和纳米材料领域的应用正在逐步扩大。近年来，酶与聚合物的结合已成为检测生物分子的重要手段。本文综述了酶介导聚合的机理和发展历程，讨论了酶介导控制聚合在生物分子分析中的应用，以及模拟酶和固定化酶在酶促聚合中的重要性。最后，总结了酶促聚合的一些新趋势和重要进展，以及酶促聚合在生物分子分析中的应用面临的主要挑战和未来前景。此外，本文还着重介绍了酶聚合技术在生物传感器领域的应用。

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来源期刊

Microchemical Journal 化学-分析化学

CiteScore

8.70

自引率

8.30%

发文量

1131

审稿时长

1.9 months

期刊介绍： The Microchemical Journal is a peer reviewed journal devoted to all aspects and phases of analytical chemistry and chemical analysis. The Microchemical Journal publishes articles which are at the forefront of modern analytical chemistry and cover innovations in the techniques to the finest possible limits. This includes fundamental aspects, instrumentation, new developments, innovative and novel methods and applications including environmental and clinical field. Traditional classical analytical methods such as spectrophotometry and titrimetry as well as established instrumentation methods such as flame and graphite furnace atomic absorption spectrometry, gas chromatography, and modified glassy or carbon electrode electrochemical methods will be considered, provided they show significant improvements and novelty compared to the established methods.